U3A1: 4A endonucleases, ligases and polymerases Flashcards

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1
Q

role of endonuclease

A

cut DNA by splitting the phosphodiester bond of the sugar-phosphate backbone.

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2
Q

circular DNA v linear DNA

A

circular DNA: no. fragments = no. recognition sites
linear DNA: no. fragments = no. recognition sites +1

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3
Q

role of ligase

A

joins two fragments of DNA or RNA together. ligase can join both sticky and blunt ends.

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4
Q

role of polymerase

A

adds nucleotides to DNA or RNA.

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5
Q

primary role of RNA polymerase

A

transcription of genes

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6
Q

primary role of DNA polymerase

A

replication/amplification of DNA

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