KA1: laboratory techniques for biologists: detecting proteins using antibodies Flashcards
what are immunoassay techniques used to detect and identify?
specific proteins
what do immunnoassay techniques use?
stocks of antibodies with the same specificity, known as monoclonal antibodies
what is an antibody specific to the protein antigen linked to?
a chemical “label”
what is this “label”?
a reporter enzyme producing a colour change c
chemiluminescence, fluorescence and other reporters can be used
in some cases, what does the assay use to detect the presence of antibodies?
a specific antigen
what is western blotting?
technique used after SDS–PAGE electrophoresis
describe the use of western blotting
The separated proteins from the gel are transferred (blotted) onto a solid medium
The proteins can be identified using specific antibodies that have reporter enzymes attached
