Vitamin C Flashcards
Vitamin C is an ______ vitamin
essential; has to be obtain through the diet
How is vitamin C used: biological activity
+ Protein/Hormone Synthesis
+ Regeneration of AOX
+ Increases absorption of iron
How is vitamin C used: Food Industry
+ Prevent oxidation
+ Delay spoilage
+ Color retention
+ Acidic flavor
How is vitamin C used: Beauty Industry
“Anti-aging”
Biological Assays
+ Bioavailability Studies
+ Human/Animal Studies
Microbiological Assays
+ Measures quantity needed for growth
+ Time consuming
+ Measures: yeast, mold, bacteria
Extraction
+ Cold extraction
+ Reducing agents
+ Dilution
Conditions of Analysis
+ pH
+ Temperature
+ Clarity of Sample Color
+ Oxygen
Reagents
+ Boric acid sodium acetate
+ Acetic Acid
+ Beta mercaptoethanol
Methods of Detection (Vitamin C)
+ 2,6-Dichloriondophenol
+ 0-phenylene diamine
+ HPLC/UPLC
Type of Method: 2,6-Dichloriondophenol
(For what)
Titration Method
(Juices and vitamins)
Type of Method: 0-phenylene diamine
(For what)
Fluorescent Spectrometry
(Vitamin Supplements)
Type of Method: HPLC/UPLC (liquid chromatography)
(For what)
Separation Technique +Mass Spectrometry
(Infant formulas and nutritional drinks)
