Lesson 2.3: Oxygen Requirements Flashcards
Oxygen relationships of microorganisms
Obligate Aerobes
require oxygen
aerobic respiration
Micrococcus luteus (skin, dust)
Oxygen relationships of microorganisms
Facultative Anaerobes
do not require oxygen, but grows better with it
aerobic and anaerobic respiration, fermentation
E.coli
Oxygen relationships of microorganisms
Microaerophilic
required but low levels (microoxic conditions)
Aerobic respiration
Oxygen relationships of microorganisms
Anaerobes
does not respire oxygen
Oxygen relationships of microorganisms
Aerotolerant
tolerate oxygen but do not use it
fermentation
Oxygen relationships of microorganisms
obligate anaerobes
Examples
inhibited or killed by oxygen
Clostridium- gram-pos, spore-forming
methanogens
Growth of bacteria on the medium based on their oxygen requirement
Obligate aerobes- top
Facultative anaerobes- grow throughout
Microaerophiles - grow near the top
Anaerobes- bottom
Methods of culturing for aerobic
shaking flasks or bubbling air
Methods of culturing for strict anaerobes
incubation in oxygen-free gas chamber
anoxic glove bags
remove trace oxygen