LE PREP Flashcards
1
Q
- _is an autoimmune disease in which the
immune system attacks its own tissues,
causing widespread inflammation and tissue
damage in the affected organs
A
SYSTEMIC LUPUS ERYTHEMATOSUS
2
Q
SYSTEMIC LUPUS ERYTHEMATOSUS . It can affect
the what parts of the body?
A
joints, skin, brain, lungs, kidneys, and
blood vessels
3
Q
an antibody found in the serum
especially of patients with systemic lupus
erythematosus
A
LE factor
4
Q
A
5
Q
neutrophil containing a large
spherical body in its cytoplasm
A
LE cell
6
Q
specimen used for LE prep
A
heparinized blood
7
Q
materials used for LE prep
A
- Glass beads
- Mechanical rotator
- glass tubes
- Glass slides
- Wright Stain
- Dropper
- Water or dry bat
8
Q
principle of LE prep
A
principle:
addition of glass beads to break neutrophil to expose the nucleus to be engulfed the macrophage
9
Q
procedure for LE prep
A
- Add 5-10 glass beads to the tube of blood and mix on a mechanical rotator for 30 minutes
- Incubate tube of blood at 37C for 30 minutes
- Fill 3 to 4 Wintrobe tubes with the well mixed blood and centrifuge at 200g for 10 minutes
- Discard the plasma from the tubes. Remove the buffy coat and prepare 3 to 4 smears. Stain with Wright stain.
- Examine each smear for the presence of LE cells under OIO. Report results as positive or negative.
- The slides should be examined for at least 10 minute
10
Q
A smear is considered
positive when ___
A
10 or more
characteristic LE cells are
seen
