W2 - Post Translational Modifications

Question 1

PTMs are considered a part of synthesis. True or false?

Answer 1

False

Question 2

What 3 amino acids can be phosphorylated? (OH on side chain)

Answer 2

Serine, threonine and tyrosine

Question 3

Is phosphorylation done in prok/euk/both?

Answer 3

Both

Question 4

What enzymes add/remove phosphates?

Answer 4

Kinase adds (highly selective), phosphatase removes

Question 5

How can phosphorylation change the conformation + activity of proteins?

Answer 5

PO4^2- adds 2-ve charges

Question 6

Where are the two locations that acetylation can occur?

Answer 6

N-terminal of an aa and @ Lysine residues

Question 7

What actions occur @ histones @ lysine residues to increase/decrease transcriptional activity?

Answer 7

Increase - acetylation (2 -ves repel), decrease - deacetylation

Question 8

What’s the function of acetylation @ the N-terminal of an aa?

Answer 8

Protection from aminopeptidases, increasing the half life

Question 9

Is acetylation done in prok/euk/both?

Answer 9

Euk ONLY

Question 10

What is the enzyme that moves an acetyl group?

Answer 10

Acetyl transferase

Question 11

Is glycosylation in prok/euk/both?

Answer 11

Euk ONLY

Question 12

Where in eukaryotes does glycosylation take place?

Answer 12

Lumen of ER and GA