Molecular Biology Flashcards
What is xenobiotics?
Chemicals that are foreign to a cell.
Why is cell membrane not permeable to ions?
It’s non-polar + like dissolves like.
What do you understand by miscible and ionophores?
Ionophore are mixable with plasma membrane hence can deliver ions into the cell cytoplasm in various ways.
What are the types of signaling molecules?
Two types (hydrophobic and hydrophilic)
What are the types of signaling molecules with cells?
3 (ion channel proteins, enzyme based proteins and g protein based signaling systems)
What is an oncogene?
A cancer causing gene.
What do you understand by cell signaling?
Can be achieved in three ways (ionic channels, enzyme or g protein based cell signaling)
Why is plasma membrane asymmetric?
Different distribution of protein, lipids, and carbohydrate etc. between outer and inner leaflets.
True or False? Glucolipids are present outside and inside on the plasmalemma?
False! They’re only present on the outer leaflet towards the extracellular environment.
Other than being embedded permanently inside the plasma membrane what other types of integration can exist for integral proteins?
They can be embedded inside the plasma membrane via fatty acyl or prenyl etc. groups. Not necessarily completely embedded within the membrane in all scenarios.
What are the types of Transportation in cell?
Passive(simple and facilitated diffusion( gated and ungated channels (gated=mechanical, gprotein, and ligand) + Active(Ionic channels, catalytic channels), ionophores.
Gives names of Signaling systems.
Hydrophilic (neurotransmitters) and hydrophobic (hormones) molecules + receptors(catalytic, ionic, g-protein coupled receptors).
What is ankyrin and vinculin? Where are they found and what’s their function?
Ankyrin is the cytoskeleton structure of RBC and Vinculin is ////// of Non erythroid cells. Both bind with integrin protein.
What receptor type does the epinephrine hormone binds to?
G-protein coupled receptors (beta and alpha 2 adrenergic receptors)
Where is rRNA and ribosome present in the nucleus?
Synthesized and present within the nucleolus.
What is euchromatin and heterochromatin?
Transcriptionally active and inactive versions.
What is karyotype?
The number and morphology of chromosomes in an organism.
Name the four proteins associated with DNA nucleosomes and folding.
H2A, H2B, H3, and H4
Which DNA protein is involved in condensed chromatin?
H1
Name the start and end codons.
Start(AUG), Stop(UAG, UGA, and UAA).
Name the enzymes which synthesize mRNA, rRNA, and tRNA?
RNA polymerases 1(rRNA) 2(mRNA), and 3(tRNA)
What do you understand regarding miRNAs?
Inactive form of RNA, suppress mRNA translation hence gene expression. Candidate for cancer treatment.
What parts make up the large and small subunit of the ribosome?
45s(28, 5.8. and 5S) - Large subunit
18s from 45s makes the small subunit of the chromosome.
Name the cyclins involved in cell cycle and their role?
Cyclin kinases (A,B,D,E). D and E help in progression s phase advancement, A helps in G2 advancement whereas B transition into the M-phase.
What’s the difference between chromatids and chromosomes?
Both are the same thing with exception that chromatids are chromosomes that will go into individual daughter cells after mitosis. Chromatids appear only at the time of cell-division.
How would a cell division get arrested at G1 stage?
If the mother centrioles do not move to the intracellular bridge from the newly developing poles.
What enzyme is crucial to apoptotic DNA cleavage?
Caspases or CAD
Why is chromatin condensation important during apoptosis?
Makes the linker DNA portion of nucleosomes more accessible to CAD. Can happen without condensation but it’s less efficient.
What factors can trigger apoptosis?
Gene activation or TNF
What are the main events in prophase 1 of meiosis?
Synapsis and tetrad formation, crossing over at chiasmata.
What are the phases of prophase 1 of meiosis?
Leptotene, zygotene, pachytene, diplotene, and diakinesis.
What do you understand by homologous chromosomes?
Two pairs of chromosomes, one from each parents. Same size and same gene location.
What are the main events of the 5 stages of prophase 1 of meiosis?
Leptotene( condensation), zygotene(synapsis), pachytene(crossing over), diplotene(Tetrad dissolution), Diakinesis( disappearance of nucleolus, nuclear membrane breakdown and end of chiasmata).
Crossing over takes place between what chromatids?
Non sister homologous chromatids.
What do you understand by synaptonemal complex and tetrads?
They are protein RNA complex attaching matched homologous chromosomes whereas tetrad is the complex formed after synapsis of chromatids. Tetrad can exist without synaptonemal complex vice versa not possible.
What is Ribophorins and where are they found?
They’re receptors for large ribosomal subunits and are present in RER.
The enzymes of TCA cycle or Krebs cycle are located in which organelle?
Mitochondria
Where is the ETC system present in cell. What other mechanics are used by the organelle to produce energy in the form of ATP
They’re present in the inner membrane of mitochondria. Other process involves oxidation of fatty acids, amino acids, and glucose.
What do you understand by condensed mitochondria?
Present in brown fat cells, produce heat instead of ATP, inner membrane is smaller with high matrix density, whereas intermembrane space is larger.
What is the main function of golgi apparatus
Processing protein packages from RER + redistribution and recycling of membranes
What do you know about the cis, trans, and VTC?
cis is the face of Golgi apparatus facing the RER and nucleus whereas trans is the face of the organelle towards the plasma membrane. VTC is a cisternae between Golgi and RER.
What is Cristae, Cisterna, and Cisternae?
Cristae(mitochondrial folding), Cisterna, and cisternae are the grooves/ saccules or inner channels of RER and Golgi apparatus respectively.
What organelle mediates the uncoupling of receptors and ligands during the endocytosis to recycle these materials?
Endosomes.
Name the types of vacuole attachment with endosome.
Autophagic vacuoles, and phagocytic vacuole.
What type of endosome fuses with the vacuoles?
Late endosome.
What organelles supply the endosome with lysosome enzyme and membrane?
RER synthesizes the necessary proteins, Golgi packages them and supplies the membrane and hydrolase enzymes in separate vacuoles to the endosomes.
What are peroxins?
The membrane of peroxisome contains special receptors called peroxins which aids in importing cytosolic proteins for peroxisome synthesis.
This organelle gets synthesized by a pre-existing organelle.
Peroxisomes
What is an inclusion in cell biology? What inclusions lack membrane?
They are materials which are temporarily within the cytosol. Glycogen and lipid droplets are not bounded by a membrane.
What’s the relationship between centrosome and microtubule?
Microtubule is a cytoskeletal structure whereas centrosome is the organelle which provides platform to microtubules for spindle fiber formation during cell division. Centrioles are present for organization purpose in centrosomes only.
What is the structure of microtubules? What proteins help in movement and attachment with organelles?
Made of tubulin, MAPS binds with cytoskeletons and organelles whereas kinesin and dynein help in organelle related movement.
Explain the polymerization and depolymerization of microtubules and actin filament cytoskeletons.
In both cases takes place at the + end of the filaments, GTP attaches in case of microtubules and ATP in case of actin filaments.
Explain the uptake and release of substances across a cell.
Endocytosis(Receptor mediated, pinocytosis, or phagocytosis) Exocytosis(
Why is clathrin important?
It’s a coating formed around a vesicle after cell endocytosis. Recycled
Explain the requirements of exocytosis?
Attachment of secretory receptors and plasma membrane + adhesion or joining of the p bilayer.
Explain the initiation step of protein synthesis?
Small ribosome subunit combines with mRNA, methionine tRNA combines with the codon on mRNA at site p of the small subunit. After this the large subunit combines to start the process of protein synthesis.
What type of tRNA is used in the protein synthesis?
Acylated tRNA
What happens at the three sites of the ribosome, namely A,P, and E?
A = New tRNA comes here
P = initiator tRNA is here (peptide bond formation and chain grows here)
E = tRNA leaves from this site
What is signal sequence?
Special codon which encodes for signal sequence, this sequence helps the newly formed peptide to enter the RER cisterna.
Explain the process of signal sequence?
- mRNA synthesis of signal sequence
- SRP binds to SS.
- SRP relocates polysomes(multi-ribosomes) to a SRP receptor on RER.
- Large subunit attaches to ribosome receptor protein, SRP detaches.
- Multisubunit protein translocators form pore on RER, synthesis continues and new polypeptides enters the cisterna through this pore.
What vesicles takes the polypeptide from RER to the cis golgi sacs?
coatomer-2 vesicles
What vesicles bring back polypeptides that escaped from the RER.
Coatomer 1 vesicles
Where does retrograde and anterograde takes place?
VTC
What are the three ingredients of ground substance?
Glycosaminoglycans (GAGs), Proteoglycans, and glycoproteins.