RP1-rate of enzyme controlled reaction

Question 1

What are the four factors affecting enzyme action

Answer 1

-temperature
-pH
-substrate conc.
-enzyme conc.

Question 2

How can a control be set up for measuring enzyme activity

Answer 2

-use distilled water or boiled enzyme solution instead of regular enzyme solution

Question 3

How can the results of this experiment be used to find the rate of the reaction

Answer 3

-plot graph of rate of reaction against time
-draw a tangent at desired time

Question 4

Outline the practical procedure of measuring enzyme activity with trypsin and milk

Answer 4

-immerse equal volumes of trypsin and milk in a water bath for 5 mins
-mix together and immediately start a timer and record time taken for solution to be fully hydrolysed (becomes colourless)
-test at 5 different temperatures with at least 3 repeats

Question 5

how is rate of reaction calculated

Answer 5

1/time

Question 6

what is the effect of temperature on enzyme activity

Answer 6

• as temperature increases, kinetic energy increases so more enzyme substrate complexes form so rate of reaction increases
  -past optimum temp, bonds in tertiary structure break and changes shape of active site
  -substrate no longer hydrolysed by denatured enzyme, rate decreases

Question 7

What is a risk of this practical and a precaution

Answer 7

-some may have allergic reactions the enzymes
-avoid contact with skin and wear goggles