stable isopotes and Mass Spec and muscle protein turnover (PRACTICAL)

Question 1

what is the basic principle of mass spectrometry

Answer 1

understanding how much tracer/label has been given (Precursor) and how much has gone into the protein (product)

Question 2

how does Gas chromatography separate complex mixtures?

Answer 2

• by increasing the temperature of “oven” so the compound becomes more volatile and is pushed through the column by the helium flow

Question 3

what does the mass spectrometer do and what 2 things does this allow for?

Answer 3

• fragments compound
• allows you to identify the compound using a quadropole analyzer
• allows you to measure the amount of tracer (eg: 13C) in the compound

Question 4

what 3 things is GC-MS relevant to in the real world?

Answer 4

• drug abuse
• doping in sport
• human metabolic research (Protein, fat, carbohydrate metabolism)

Question 5

what does muscle full mean?

Answer 5

despite continued availability of amino acids, MPS responses are time-limited

Question 6

define anabolic resistance/blunting

Answer 6

MPS responses to protein nutrition are blunted with advancing age, inactivity and some diseases states even with ENHANCED doses

Question 7

**what are the 2 mass spectrometry techniques used to study muscle protein synthesis?

Answer 7

• GC-MS
• GC with IRMS

Question 8

define concentration

Answer 8

amount of a compound in a given volume

Question 9

define enrichment

Answer 9

the amount of heavy/stable isotope present in a compound

Question 10

how can you dilute enrichment and what does this do to concentration?

Answer 10

• by adding more unlabeled compound
• this increases concentration