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Shelf 2014 > Micro - Toxins/ Virulence Factors/ culture plating > Flashcards

Micro - Toxins/ Virulence Factors/ culture plating Flashcards

1
Q

Inactivate elongation factor (EF-2)

A

Corynebacterium Dipetheriae; Pseudomonas Auerginosa

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2
Q

Inactivate 60S ribosome by removing adenine from rRNA (prevent protein synthesis)

A

Shigella (shiga toxin); Enterohemorrhagic E coli (EHEC) including O157:H7 strain (shiga like toxin)

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3
Q

Overactivates adenylate cyclase (inc cAMP)

A

Enterotoxigenic E. Coli (ETEC); Bacillus anthracis (edema factor); Vibrio choloreae (cholera toxin–Gs); Bordetella pertussis (pertussis toxin –disabling Gi)

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4
Q

Phospholipase (lecithinase) that degrades tissue cell membranes

A

Clostridium perfingens (alpha toxin)

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5
Q

Protein that degrades cell membrane (lyses RBC)

A

Streptococcus pyogenes (streptolysin O) – diagnositic for rhematic fever

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6
Q

Overwhelming IFN-gamma and IL-2 activation –> shock

A

Staph aureus (TSST-1); Strep pyogenes (exotoxin A)

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7
Q

Most common serotype of E. Coli causing UTI

A

K1 capsule (capsule = most important virulence factor)

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8
Q

Growth at 4C selects for

A

Listeria or Yersinia enterocolitica

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9
Q

Growth at 42C selects for

A

Campylobacter

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10
Q

Growth on sorbitol MacConkey agar

A

Enterohemorrhagic E coli is the only E coli that can ferment sorbitol

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11
Q

Erythrogenic exotoxins

A

Strep pyrogenes - extra virulence factor that contributes to scarlet fever; obtained through lysogenic conversion (phage inserted into bacterial chromosome and produces a prophage)

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12
Q

Lowenstein Jensen agar

A

Mycobacterium tuberculosis

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13
Q

Cold agglutination test

A

Mycoplasma pneumoniae

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14
Q

Eosin-methylene blue agar

A

E. Coli - green metallic sheen

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15
Q

Charcoal yeast agar

A

Legionella

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16
Q

Tellurite agar

A

C. Diptheriae

17
Q

Chocolate agar with V&X

A

H. influenzae

18
Q

Lysogenic phage encoding bacterial toxin (x5)

A 
ABCDE
shigA-like toxin
Botulinum toxin
Cholera toxin
Diptheria toxin
Erythrogenic toxin of strep pyogenes (scarlet fever)
19
Q

C. Dif toxin

A

Toxin A - entertoxin, binds to brush border of the gut. Toxin B - cytotxin, causes cytokeletal disruption via actin deploymerization –psuedomembranous colitis

20
Q

Stain acid fast

A

mycobacterium; nocardia (partially); cryptosporidium; isospora. Ziehl-Neelsen Acid fast stain

21
Q

Require cysteine to grow

A

Fracisella; brucella; legionella; pasturella

22
Q

Warthing Finkeldery giant cells

A

Measles - syncytium of lymphocytes (not macrophages as in most)

23
Q

F fusion protein found in

A

Paramyxovirus: parainfluenza, mumps, measles, RSV; helps them form multinucleated giants cells with the respiratory epithelial cells

24
Q

subacute sclerosing panencephalitis associated with

A

measles

25
Q

Elek test

A

C. Diptheria - toxin not produced until infected with a temparate phage that encodes the toxin - lysogenic conversoin.
ELEK test finds the toxin (b/c C.D. is morphological similar to normal oral-pharynx flora)

26
Q

Relapsing fever

A

Boriella

27
Q

Black fly, black skin nodules, black sight…name that bug?

A

Onchocerca volvulus - river blindness.

28
Q

vitamin b12 def caused by this parasite

A

diphyllobothrium latum - tapeworm

29
Q

portal htn caused by this parasite

A

schistosoma (host=snail) (penetrates skin of human)

30
Q

3 parasites that penetrate skin of human

A

stronglyoidies, Necator americanus, schistosoma

31
Q

Hairy oral leukoplakia caused by what virus?

A

EBV

32
Q

lacy body rash (exanthema subitum)

A

HHV-6 Roseola - fever for 3 days then goes away and rash comes

33
Q

Coxiella burnetii causes what disease?

A

Q fever - high fever +/- atypical penumonia. often seen in vets and farmers. rickettsial organism.

34
Q

microaerophilic (x2)

A

campy; helio

35
Q

catalase positive (x5)

A

staph, psuedomonas, candida, aspergillus, enterobacteriaceae

36
Q

giemsa stain

A

blood protozoa (plasmodium, babesia, trypanosoma, leishmania), histo, chlamydia, rickettsiae

Shelf 2014 (6 decks)

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