Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

[MT 6319] Molecular Biology & Diagnostics Lecture > [1S] UNIT 1.1 Introduction to Molecular Biology & Diagnostics > Flashcards

[1S] UNIT 1.1 Introduction to Molecular Biology & Diagnostics Flashcards

1
Q

● Biological activity at the molecular level
● Biology and chemistry

A

Molecular Biology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

T/F: The science of Molecular Biology is very universal

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

● Polymerase chain reaction
● Molecular cloning
● Gel electrophoresis
● Macromolecule blotting and probing
● Microarrays
● Allele-specific oligonucleotide

A

Molecular Techniques

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

MACROMOLECULE BLOTTING & PROBING

Southern Blot
● Target molecule
● Sample preparation
● Separation

A

DNA
DNA extraction enzymatic digestion
Electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

MACROMOLECULE BLOTTING & PROBING

Northern Blot
● Target molecule
● Sample preparation
● Separation

A

RNA
RNA isolation
Electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

MACROMOLECULE BLOTTING & PROBING

Western Blot
● Target molecule
● Sample preparation
● Separation

A

Protein
Protein extraction
Electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

MACROMOLECULE BLOTTING & PROBING

Southern Blot
● Membrane material
● Probe
● Probe label
● Detection methods

A

● Nylon
● Nucleic acid probe with sequence homologous to target
● Radiolabel, enzyme
● X-ray, film, chemiluminescence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

MACROMOLECULE BLOTTING & PROBING

Northern Blot
● Membrane material
● Probe
● Probe label
● Detection methods

A

● Nylon
● RNA, DNA, or oligodeoxynucleotide
● Radiolabel, enzyme
● X-ray, film, chemiluminescence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q
A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTIONS

Diagnostic tests to complement traditional risk factors

A

Risk Assessment

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Implement wellness programs proactively

A

Risk Assessment

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTION

Use for definitive diagnosis and general typing

A

Diagnosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTION

Applied to high-rish patient to identify disease early

A

Screening

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Nip disease in the bud with early treatment

A

Screening

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Refer to the appropriate specialist

A

Diagnosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTION

Access severity and/or risk of recurrence
Inform adjuvant therapy decision

A

Staging and Prognosis

14
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Determine whether treatment is necessary

A

Staging and Prognosis

15
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTION

Used to predict efficacy or safety response to specific treatments

A

Therapy Selection

16
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Do not waste unproductive therapy

A

Therapy Selection

17
Q

STAGES OF MOLECULAR DIAGNOSTICS CLINICAL IMPLICATIONS

Control disease progression with changes in treatment

A

Monitoring

17
Q

4 Molecular Diagnostics

A

Diagnostic - better/faster
Prognostic - more powerful
Predictive - preventive
Therapeutic - more personalized

17
Q

STAGES OF MOLECULAR DIAGNOSTICS DESCRIPTION

Recurrence monitoring
Monitoring for treatment efficacy

A

Monitoring

18
Q

4 Applications for Molecular Biology

A

● Molecular Genetics
● Infectious Disease
● Molecular Oncology
● Theragnostics

18
Q

MOLECULAR GENETICS

susceptibility test usually aiming at a low risk population

A

Genetic Screening

19
MOLECULAR GENETICS analysis of DNA or biomarkers for the evaluation of one or more genetic risk factors for a particular disease or disease group.
Genetic Testing
20
20
These are pathogens commonly identified using ______ ● Respiratory viruses - COVID ● Herpesviruses ● Clostridium difficile ● Staphylococcus aureus ● Methicillin-resistant S. aureus (MRSA) ● Streptococcus pyogenes ● Streptococcus agalactiae ● Bordetella pertussis
Nucleotide Acid Amplification Test (NAATS)
21
What kind of infections are the ff ● Neisseria gonorrhoeae ● Chlamydia trachomatis ● Trichomonas vaginalis ● Mycoplasma genitalium
Sexually transmitted infections
22
22
23
23
Challenges of Molecular Biology & Diagnostics
● Physical Facility / Laboratory Set-up ● Cost ● Education & Training ● Ethical, Legal and Social implications ● Quality Assurance ● Assay Development
24
24
application of genomic technology to new drug discovery and development
THERAGNOSTICS: Pharmacogenomics
25
● inherited changes in phenotype (appearance) or gene expression caused by mechanisms other than changes in the underlying DNA sequence. ● these changes may remain through cell divisions for the remainder of the cell's life and may also last for multiple generations.
Epigenetics
26
CHALLENGES ● Informed consent ● Genetic Counselling ● Confidentiality ● Genetic exclusion
Ethical, Legal, Social Implications
26
● Known genetic sequence variation ● Specific assay: primer design
Assay Development

[MT 6319] Molecular Biology & Diagnostics Lecture (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions