2. Flashcards

1
Q

How do we see bacteria?

A

We have to make slides

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2
Q

What is a bacterial smear?

A
  • Thinly spread bacteria on a slide.
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3
Q

Staining is when?

A

we dye the bacteria so that it can be seen against a background

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4
Q

What is the charge of staind

A

Stains are usually positively charged dyes that are attracted to the negatively charged nucleic acid and cell wall

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5
Q

What are the common basic stains?

A

include methylene blue, crystal violet, and carbol fuchsin

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6
Q

The steps of slides

A

1) First, we spread thinly spread some bacteria on a slide
This is called a bacterial smear

2) Then, we dye the bacteria so that it can be seen against a background
This is called staining
Stains are usually positively charged dyes that are attracted to the negatively charged nucleic acid and cell wall
Common basic stains include methylene blue, crystal violet, and carbol fuchsin

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7
Q

uses one color to help us see bacteria against the background

A

simple staining

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8
Q

help to identify and differentiate different types of bacteria

A

dfferentrial stain

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9
Q

What are the 5 differentrial stains

A

-Gram stains are a type of differential stain
-Negative Staining
-Acid-Fast Staining
-Endospore Staining

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10
Q

Bacteria have a thick cell wall made of the

A

polysaccharide peptidoglycan

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11
Q

Gram-positive bacteria color and cell wall

A
  • purple
    -Thick cell wall
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12
Q

Gram-negative bacteria color and cell wall

A

Pink and thin cell wall

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