2-6: Movement of Bacterial Cells Flashcards

1
Q

What is motility

A

The ability to propel your own movement

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2
Q

What is the flagellum

A

Large, complex, multi-protein (~50) machine that powers movement

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3
Q

How does the flagellum work

A

Long, thin filament that acts as propeller, rotated by motor anchored in cell envelope

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4
Q

What is swarming

A

Coordinated multicellular movement across a solid surface

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5
Q

What are the different flagellar arrangements

A

Peritrichous = many across body/pole
Monotrichous = single at pole
Lophotrichous = many, all at one pole
Amphitrichous = both poles
Atrichous = none

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6
Q

“runs” in peritrichous bacteria

A

Counterclockwise, flagella bundle at tail, cell moves forward

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7
Q

Rotation of short “tumbles”

A

Peritrichous only: One or more flagella rotate clockwise, therefore bundle falls apart, tumbles, assumes new random orientation

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8
Q

How do monotrichous bacteria move

A

Reversible flagellums, can rotate clockwise or CCW (runs)
OR
Unidirectional flagella, rotation stops/starts, movement during stops = direction change

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9
Q

What are the three segments of the flagella

A

1) Filament (long, thin propeller driving movement)
2) Hook (adaptor that connects filament to basal body)
3) Basal body (core of structure, powers rotation, motor is part of)

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10
Q

What drives motor rotation

A

Proton motive force

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11
Q

What are the parts of the gram negative flagellar motor

A

Central rod, MS ring, C ring, P ring, L ring, stator

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12
Q

Where are the MS, C, P and L ring located

A

MS = cm
C = cytoplasm
P = peptidoglycan
L = om

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13
Q

What does the stator do

A

couples flow of protons to rotation of MS

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14
Q

What does the MS do

A

Rotates rod, ultimately the hook and filament

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15
Q

What do the L/P rings do

A

Bearings to help rotation

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16
Q

What does the C ring do

A

Generate torque, switch motor direction, flagellin secretion

17
Q

What does gram positive flagellum lack

A

P/L rings

18
Q

What is flagellin

A

Proteins, thousands of which make up the filament

19
Q

How long and wide is the filament

A

5-10um long, 20nm wide

20
Q

Is the filament highly conserved

A

Yes

21
Q

How is flagellin detected by our immune systems

A

It is an important antigen (H antigen)

22
Q

How is flagellum made

A

Inside -> out
Produced in cytoplasm, secreted via “export apparatus” (type 3 secretion system), through thin pore in basal body.

23
Q

Variations in some bacteria flagellar motility?

A

Some use Na+ gradient, spirochetes have axial filament that resides in periplasm, rotates like a crokscrew

24
Q

What is a taxis

A

Directed movement of bacteria towards attractant

25
Q

what is chemotaxis

A

Movement in direction of gradients (increase or decrease) in concentration of a chemica

26
Q

what is chemotaxis

A

Movement in direction of gradients (increase or decrease) in concentration of a chemical
Detected by chemoreceptors

27
Q

What is phototaxis and aerotaxis

A

P = movement towards/away from light
A = directed motility in response to O2

28
Q

What is twitching motility

A

Non-flagellar
Type 4 pilus attaches to surface then retracts “grappling hook”