2: COMMENSAL AMEBAE Flashcards

1
Q

The three genera
of intestinal amebae can be differentiated
through the morphological features of their
________.

A

nuclei

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2
Q

Trophozoites usually have only ______ nucleus.

A

one

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3
Q

The genus _____________ has a vesicular nucleus with a relatively large, irregularly-shaped karyosome anchored to the nucleus by achromatic fibrils.

A

Endolimax

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4
Q

The genus ____________ is characterized by a large, chromatin-rich karyosome surrounded by a layer of achromatic globules and anchored to the nuclear membrane by achromatic fibrils.

A

Iodamoeba

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5
Q

All species have the following stages:

A

trophozoite, precyst, cyst, and metacystic
trophozoite; (with the exception of Entamoeba gingivalis)

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6
Q

has no cyst stage, and does not inhabit the intestines

A

Entamoeba gingivalis

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7
Q

Excystation occurs in the alkaline environment of the ________________________

A

lower small intestines

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8
Q

__________________colonize the large intestines and live on the mucus coat covering the intestinal mucosa

A

Metacystic trophozoites

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9
Q

____________________ is morphologically
similar to E. histolytica, but their DNA and
ribosomal RNA are different

A

Entamoeba dispar (isoenzyme pattern is different)

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10
Q

It is a non-pathogenic species
that is morphologically indistinguishable
from E. histolytica and E. dispar, but differs
from them biochemically and genetically

A

Entamoeba moshkovskii i

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11
Q

E. moshkovskii is also physiologically unique—it being _______________ , able to grow at room temperature (25-30°C optimum), able to survive at temperatures ranging from 0 to 41°C.

A

osmotolerant

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12
Q

All human isolates have been found
to belong to one group___________.

A

“ribodeme 2

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13
Q

The appearance of _____________ is relatively
similar to that of E. histolytica apart from its
smaller size.

A

E. hartmanni

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14
Q

_____________ does not ingest red blood cells.

A

E. hartmanni

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15
Q

______________ is cosmopolitan in
distribution, and is considerably more common than other human amebae.

A

Entamoeba coli

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16
Q

E. coli can be differentiated from E. histolytica trophozoite by the following features:

A

1) a more vacuolated or granular endoplasm with bacteria and debris, but no red blood cells;
2) a narrower, less-differentiated ectoplasm;
3) broader and blunter pseudopodia used more for feeding than locomotion;
4) more sluggish, undirected movements; and
5) thicker, irregular peripheral
chromatin with a large, eccentric karyosome in the nucleus

17
Q

An E. coli cyst may be differentiated from
E. histolytica by:

A

1) its larger size (10 to 35 μm in diameter),
2) more nuclei (eight versus four in E. histolytica),
3) more granular cytoplasm, and
4) splinter-like chromatoidal bodies

18
Q

__________________ is a parasite found in
the intestines of pigs and monkeys.

A

Entamoeba polecki

19
Q

E. polecki can be distinguished from E.
histolytica in that the former’s cyst is:

A
  • consistently uninucleated
  • chromatoidal bars are frequently angular or pointed.
20
Q

__________________, which is found in apes
and monkeys, is morphologically identical to
E. polecki.

A

Entamoeba chattoni

21
Q

Identification of E. chattoni was done via ______________

A

isoenzyme analysis

22
Q

___________________ can be found in the mouth.

A

Entamoeba gingivalis

23
Q

It moves quickly, and has numerous
blunt pseudopodia.

A

Entamoeba gingivalis

24
Q

________________ lives on the surface of
gum and teeth, in gum pockets, and sometimes in the tonsillar crypts. They are abundant in cases of oral disease.

A

E. gingivalis

25
This species has no cyst stage. Transmission is most probably direct: through kissing, droplet spray, or by sharing utensils.
E. gingivalis
26
__________________ occurs with the same frequency as Entamoeba coli. Trophozoites are small, with a diameter of 5 to 12 μm, and exhibit sluggish movement. They have blunt, hyaline pseudopodia, and the nucleus has a large, irregular karyosome. Food vacuoles found in the cytoplasm may contain bacteria. Cysts measure about the same size as trophozoites, and are quadrinucleated when mature
Endolimax nana
27
It is identified by its characteristic large, vesicular nucleus with a large, central karyosome, surrounded by achromatic granules. There are no peripheral chromatin granules on the nuclear membrane.
Iodamoeba bütschlii
28
Diagnosis is done through _________________.
stool examination
29
Diagnosis for E. gingivalis
a swab between the gums and teeth is examined for trophozoites
30
Trophozoites are best demonstrated by __________________.
direct fecal smear
31
In recovering cysts, the use of _____________________________ is useful
concentration techniques like FECT and zinc sulfate flotation