2.1. RNA INTERFERENCE (RNAi) Flashcards Preview

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Flashcards in 2.1. RNA INTERFERENCE (RNAi) Deck (17)
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1
Q

RNAi

A
  1. RNA interference

2. Process of sequence-specific (homology-dependent) gene silencing initiated by double-stranded RNA (dsRNA)

2
Q

miRNA

A

MicroRNA

3
Q

Pri-mRNA

A
  1. Primary miRNA

2. Large RNA

4
Q

Monocistronic

A

Single hairpin loop

5
Q

Polycistronic

A

Multiple hairpin loops

6
Q

Pre-miRNA

A

Precursor miRNA

7
Q

Drosha

A
  1. dsRNA-binding partner (DGCR8)
  2. Process Pri-miRNA to pre-miRNA
  3. Drosha + DGCR8 => microprocessing complex
8
Q

Exportin-5

A

XPO5

9
Q

Dicer

A
  1. dsRNA-binding partner TRBP

2. Process pre-miRNA to miRNA duplex

10
Q

RISC

A

RNA Induced Silencing Complex

Contains Ago-2

11
Q

Mature miRNA/ guide

A

Guides complex to complementary mRNA Wh I his silenced

12
Q

Perfect match

A

Silencing occurring through the degradation of mRNA

13
Q

Imperfect match

A

Inhibition of translation

14
Q

Downregulation

A

Target oncogene

15
Q

Upregulation

A

Target a tumor suppressor

16
Q

dsRNA

A

Double-stranded RNA initiating RNA interference

17
Q

What are the two functions of viruses and miRNA?

A
  1. Viral miRNA shown to inhibit cellular factors involved in innate/ adaptive immunity
    - human cytomegalovirus
    - Kaposi’s Sarcoma Associated herpesvirus
    - Epstein-Barr virus
    • downregulate natural killer cell Logan MICB
    • Epstein-Barr virus encoded miRNA also downregulate Pri-apoptotic protein PUMA
  2. Other viral miRNA downregulate expression of viral proteins
    - Herpes simplex virus 1 maintains latent state by down-regulating immediate - early trans activators (ICPO & ICP4)