Flashcards in 2.1. RNA INTERFERENCE (RNAi) Deck (17)
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1
Q
RNAi
A
- RNA interference
2. Process of sequence-specific (homology-dependent) gene silencing initiated by double-stranded RNA (dsRNA)
2
Q
miRNA
A
MicroRNA
3
Q
Pri-mRNA
A
- Primary miRNA
2. Large RNA
4
Q
Monocistronic
A
Single hairpin loop
5
Q
Polycistronic
A
Multiple hairpin loops
6
Q
Pre-miRNA
A
Precursor miRNA
7
Q
Drosha
A
- dsRNA-binding partner (DGCR8)
- Process Pri-miRNA to pre-miRNA
- Drosha + DGCR8 => microprocessing complex
8
Q
Exportin-5
A
XPO5
9
Q
Dicer
A
- dsRNA-binding partner TRBP
2. Process pre-miRNA to miRNA duplex
10
Q
RISC
A
RNA Induced Silencing Complex
Contains Ago-2
11
Q
Mature miRNA/ guide
A
Guides complex to complementary mRNA Wh I his silenced
12
Q
Perfect match
A
Silencing occurring through the degradation of mRNA
13
Q
Imperfect match
A
Inhibition of translation
14
Q
Downregulation
A
Target oncogene
15
Q
Upregulation
A
Target a tumor suppressor
16
Q
dsRNA
A
Double-stranded RNA initiating RNA interference
17
Q
What are the two functions of viruses and miRNA?
A
- Viral miRNA shown to inhibit cellular factors involved in innate/ adaptive immunity
- human cytomegalovirus
- Kaposi’s Sarcoma Associated herpesvirus
- Epstein-Barr virus
• downregulate natural killer cell Logan MICB
• Epstein-Barr virus encoded miRNA also downregulate Pri-apoptotic protein PUMA - Other viral miRNA downregulate expression of viral proteins
- Herpes simplex virus 1 maintains latent state by down-regulating immediate - early trans activators (ICPO & ICP4)