2.2- Magnification And Calibration

Question 1

What is magnification and how do you calculate it?

Answer 1

How many times larger the image is than the actual size if the object.
Objective lens allows users to adjust magnification.

Magnification = image size / actual size

Question 2

What is an eyepiece graticule?

Answer 2

A glass disc marked with a fine scale of 1-100 (no units).

-the scale remains unchanged if objective lens changes but the relative size of divisions increases with magnification.

The scale on the graticule at each magnification Is calibrated using a stage micrometer.

Question 3

What is a stage micrometer?

Answer 3

A slide with an accurate micro metres (um) scale engraved in it.

The scale marked on the micrometer is usually 100 divisions= 1mm.
So 1 division = 10um