2.3 Gram Staining Flashcards
1
Q
Is gram stain simple or differential?
A
differential
2
Q
In gram staining, what types of cells are differentiated?
A
Gram-positive
Gram-negative
3
Q
How old should the cultures we use be?
A
16-18 hours
4
Q
Should we prepare thick or thin smears!
A
Thin
5
Q
The most critical step in gram-staining
A
Decolorization
6
Q
Step 1: Cover a heatfixed smear with what? For how long?
A
Crystal violet
30 seconds
7
Q
Step 1: Cover a heatfixed smear with what? For how long?
A
Crystal violet
30 seconds
8
Q
Step 3: Cover the smear with what? For how long?
A
Gram’s iodine solution
1 minute
9
Q
Step 4: How should you hold the slide? What will you apply? For how long?
A
45 degree angle
Decolorizer
15 seconds
10
Q
Step 6: Cover the smear with what? For how long?
A
Safranin
1 minute
