Tools in Genetics Flashcards
What is a general advantage of genome-wide association studies compared to candidate gene studies?
A. It is cheaper.
B. It can provide a deeper understanding of a gene.
C. It is more likely to illicit the discovery of new gene/disease association.
D. It is more accurate because it studies less experimental loci.
C
Which of the following is LEAST influential in the recent progress of molecular biology?
A. Increase in scientific prizes
B. Molecular databases
C. High-throughput technologies
D. Development of better analytical software
A
Which of the following do NOT contribute novel knowledge to the medical field?
A. The use of whole genome microarray to determine potential markers for a disease
B. The use of quantitative PCR to determine the expression of genes during a diseased state
C. The use of sequencing to determine changes in microbial profile during obesity
D. The use of ELISA to test a patient for HIV
D
Which of the following do NOT use an animal model?
A. The use of rabbits to determine the toxicity of drugs through molecular markers
B. The use of the malaria parasite to make a vaccine against malaria
C. The use of the fly to study the signaling pathways of development
D. The use of genetically-engineered pigs to study the function of a gene
B
Which is TRUE of diagnostics involving nucleic acid amplification (e.g PCR)?
A. Copies of the target sequences are amplified to make detection easier.
B. Real-time PCR employs a strategy of amplifying proteins from original RNA samples.
C. Isothermal strategies are not feasible as a diagnostics in remote areas.
D. Only DNA can be amplified.
A
A physician got blood samples from an aspiring overseas Filipino worker for hepatitis B virus testing. Unfortunately, the results showed high titers for hepatitis DNA. She was prescribed to take some medications and advised to return for repeat HBV DNA determination. Which of the following is TRUE about the use of this testing?
A. It cannot detect acute infection.
B. It can elucidate the mechanism of action of the infection.
C. It can localize the infected cells.
D. It can monitor the progress of treatment.
D
Which is a general advantage of microscopic evaluation (immunochemistry) over total protein immunoassay of solid tissues from human?
A. It is more sensitive (detects more true positives).
B. It is more specific (detects more true negative).
C. It can co-localize proteins to their cells.
D. It is less time-consuming.
C
What is an importance of diagnostics that correlate the genotype of patients in response to a drug?
A. To determine the cost of the drug during marketing
B. To determine if the drug is effective in the general population
C. To identify vulnerable individuals to the side effects of the drug
D. To do a genome-wide study to determine avariability
C
Which of the following statements is TRUE?
A. Both Western blot and immunochemistry can localize
proteins in tissues.
B. Quantitative PCR is useful for both amplification and
diagnosis.
C. Electrophoresis is utilized solely for research purposes.
D. Gene sequencing often explains the cause of a disease.
B
You are interested in making a diagnostic for dengue – a cell-invasive pathogen. Which of the ff. is least likely one to consider?
A. Quantitative PCR - dengue
B. Immunoassay - host response
C. Western blot - dengue
D. Genomic sequencing - host response
D
You are interested in discovering something new about diseases. Which of the following is the correct matching in order to achieve your objective?
A. Southern Blot - to explore the presence of an unknown
pathogen
B. Xray crystallography - discover new genomic variants
associated with the disease
C. Microarray - to explore pathways associated with the
disease
D. Genomic sequencing - to discover new proteins involved
in a disease
C
Which of the following refers to genomics?
a. Cost-benefit analysis of genes
b. Global analysis of chromosomes
c. Mapping, sequencing and characterization of genes
d. Sequencing and analysis of mitochondrial
e. Study of unusual gene-gene interactions genes
c
