2ND PRELIM

Question 1

A growth of microbes

Answer 1

Culture

Question 2

Microbial population

Answer 2

Colony

Question 3

When a colony is consist of only one type of cell it is called

Answer 3

Pure culture

Question 4

3 classification of media

Answer 4

Physical state
Chemical composition
Functional type

Question 5

What are the tree physical state

Answer 5

Liquid
Semi-solid
Solid

Question 6

These media are commonly termed as broth, milk, infusion

Answer 6

Liquid media

Question 7

Exhibits a clot like consistency

Answer 7

Semi-solid media

Question 8

What are the two solidifying agents?

Answer 8

Agar and gelatin

Question 9

Provides firm surface

Answer 9

Solid media

Question 10

2 forms of solid media

Answer 10

Reversible
Non-reversible

Question 11

Contain mixture of nutrients that support pathogen and non-pathogen growth

Answer 11

General purpose media

Question 12

Designed to increase the number of microbes without stimulating the rest of the bacterial population

Answer 12

Enrichment media

Question 13

Contains agent that inhibit the growth of a certain microbe and thereby only a certain microbe can grow

Answer 13

Selective media

Question 14

Various type of microorganisms can grow it is designed to display visible differences of microorganisms

Answer 14

Differential media

Question 15

Colonies range from pinpoint(less than 1mm) to as large as 5-10mm in diameter. Only well-separated colonies should be measured since they tend to have larger diameter when crowded.

Answer 15

Size

Question 16

The periphery of the colonies may be entire, with rounded projections, notched, threadlike, filamentous, serrated, or lobate

Answer 16

Margin

Question 17

Classified as smooth(shiny, glistening), rough (dull, granular), mucoid (slimy or gummy), wrinkled, concentric, contoured and etc., It can serve as indicator of virulence.

Answer 17

Surface texture

Question 18

Can be flat, raised or convex

Answer 18

Elevation

Question 19

Can be determined by touching the colony with transfer with a transfer needle. Classified as butyrous (butterlike), viscous, stringy, dry, brittle, powdery consistency.

Answer 19

Consistency

Question 20

Colonies may be transparent, translucent, or opaque.

Answer 20

Optical features

Question 21

Differential isolation and presumptive identification of Group D streptococci and enterococci

Answer 21

Bile esculin agar

Question 22

Determination of hemolytic reactions

Answer 22

Blood agar

Question 23

Isolation of Bordatella pertussis (whooping cough) commonly used – colonies resemble mercury droplets

Answer 23

Bordet-Gengou agar

Question 24

Enrichment medium for Legionella species

Answer 24

Buffered Charcoal-yeast extract agar (BCYE)

Question 25

Selective medium for Campylobacter species

Answer 25

Campy blood agar

Question 26

Cultivation of Haemophilus species and pathogenic Neisseria species

Answer 26

Chocolate agar (CHOC)

Question 27

Selective isolation of gram-positive cocci

Answer 27

Columbia colistin-nalidixic agar

Question 28

Isolation and differentiation of lactose-fermenting and non-lactose fermenting enteric bacilli

Answer 28

MacConkey Agar

Question 29

Selective isolation of staphylococci

Answer 29

Mannitol salt agar

Question 30

Selective for Neiserria gonorrhea

Answer 30

New York city agar

Question 31

Enrichment medium for the isolation of Salmonella species

Answer 31

Selenite broth

Question 32

Selective for N. gonorrheae and N. meningitidis

Answer 32

Thayer martin agar

Question 33

Selective and differential medium for Vibrio species

Answer 33

Thiosulfate - citrate bile salt sucrose agar