final lecture Flashcards

1
Q

serum

A

the liquid portion of blood after clotting has occured

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2
Q

plasma

A

the liquid portion of blood without the cells when blood is spun down in a tube with anticoagulant

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3
Q

what is the primary function of the anticoagulant sodium fluoride?

A

antiglycolytic agent

inhibits glycolysis

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4
Q

choice of anticoagulant for hematology

A

EDTA

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5
Q

choice of anticoagulant for coagulation

A

sodium citrate

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6
Q

choice of anticoagulant for chemistry

A

heparin

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7
Q

why is freezing whole blood not normally recommended to preserve a specimen

A

the red cells will lyse causing hemolysis

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8
Q

what are the two primary reasons patients are placed in isolation rooms?

A

to protect healthcare staff and visitors from infections

to protect an immunocompromised patient

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9
Q

how does EDTA prevent clotting?

A

by binding/chelating calcium in the form of potassium or sodium salt

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10
Q

how does heparin prevent clotting?

A

inhibits conversion of prothrombin to thrombin

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11
Q

what are the capillary puncture sites on an adult?

A

the index, middle, or ring finger.

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12
Q

where would you not want to perform capillary puncture on adult?

A

the middle of the finger

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13
Q

what is the preferred area on a finger for puncture?

A

perpendicular to the fingerprints on the outside of the finger

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14
Q

what is a hematoma and what causes it?

A
  • collection of blood under the skin - indicative of poor technique
  • going through a vein
  • partially inserting needle into vein
  • grazing side of the vein
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15
Q

state the consequences of pushing a tube onto the needle before entering the patient’s vein.

A

loss of vaccum = no suction in tube

could contaminate tube

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16
Q

explain the purpose of removing the tube from the needle prior to withdrawing from the patient’s vein.

A

to release the vaccum in the tube

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17
Q

what are clot activators and some examples

A

substance that inhibits or enhances coagulation

ex. glass/silica/siliceous earth/ thrombin

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18
Q

explain the order of draw

A

the order of draw is the list of when to draw specific tubes in a specific order

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19
Q

why does the order of draw exist?

A

this exists to prevent the cross contamination of additives between tubes

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20
Q

what does a sclerosed vein feel like when palpated?

A

hard/cord like

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21
Q

should a sclerosed vein ever be used for veinipuncture?

A

no

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22
Q

what is edema?

A

swelling

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23
Q

what is fistula?

A

abnormal connection between artery and vein

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24
Q

why are fistulas important to consider when deciding on site selection for venipuncture?

A

they are used for dialysis patients, so cannot be used

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25
Q

explain what a bevel is & what abnormalities to look for

A

bevel is the slanted tip on hollow venipuncture needle

ex. bent/curved/shaved/burns/spurs

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26
Q

why are therapeutic drug monitoring tests often timed collection tests?

A

to measure the peak and trough levels because people metabolize drugs at different rates and to monitor the amount of drugs at certain times to maximize effectiveness and prevent toxicity

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27
Q

what is the name of the heel bone?

A

calcaneus

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28
Q

what does skin puncture blood most closely resemble?

A

arterial blood

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29
Q

explain why routine or strict isolation is used.

A

patient is isolated to prevent the spread of a communicable disease

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30
Q

what is the PPE used in routine and strict isolation?

A

gown, masks, gloves

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31
Q

explain protective or reverse isolation.

A

patient is isolated to protect from infection (severely immunocompromised patients)

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32
Q

what PPE is used in protective or reverse isolation?

A

gown, masks, gloves, hair net, booties

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33
Q

what does an abnormal retraction of a blood vessel wall during venipuncture cause?

A

vein collapse

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34
Q

what is the most important thing you should do before performing any type of specimen collection on a patient

A

wash hands and ID patient

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35
Q

what should you do if a patient refuses to let you collect a specimen on them?

A

let the doctor know

36
Q

why should you allow the alcohol on the skin to dry properly before inserting the needle?

A

painful to patients and will lyse red cells

37
Q

specimens should always be labeled where and when?

A

immediately after draw while still with patient

38
Q

in regards to placement of the needle’s bevel in the vein, where would it fall that it would NOT allow collection of a blood specimen?

A

anywhere except facing up
needle penetrates through entire vein
went alongside vein

39
Q

what are some possible techniques for transferring blood from a syringe into the proper collection tubes?

A

transfer device

40
Q

describe the proper puncture site on an infants’ heel for skin puncture

A

medial or lateral on plantar surface

41
Q

once a red stopper tube is spun down, what type of specimen does it yield

A

serum and clotted red cells

42
Q

how much a collection site be prepared for collecting blood cultures?

A

cleaned with beta-dyne in non-overlapping circular motion

43
Q

why is it important to take the extra steps for blood cultures?

A

to ensure sterility

44
Q

in regards to therapeutic drug monitoring, the highest concentration of the drug during a dosing interval is called the ____

A

peak

45
Q

explain the procedure for collecting specimens for a glucose tolerance test

A
  • specimen drawn after pt. has fasted overnight
  • pt. drinks glucose solution - mark time
  • specimens drawn at 1 hr. intervals until complete (up to 5 hours)
46
Q

does the order of draw change depending on the collection equipment you use ?

A

no

47
Q

why should the site be warmed before performing skin puncture?

A

increases blood flow

48
Q

why is controlling the depth of your lancet important during skin puncture?

A

if depth is not controlled could puncture bone or cause excessive bleeding

49
Q

what step must you take, when doing a skin puncture, to eliminate tissue fluid contamination?

A

wipe away first drop of blood

50
Q

what is a possible consequence of mixing a tube to vigorously?

A

hemolysis

51
Q

when drawing a blood on a child, what are some extra steps you should take prior to venipuncture because of the age of your patient?

A
  • gain child’s confidence
  • -acknowledge child and parent
  • ask for help to restrain child
52
Q

what should you not do when performing specimen collection on a child?

A
  • rush in
  • do not ignore the child
  • do not try to draw without a restrain on hand
53
Q

why should a patient not pump their fist prior to venipuncture?

A

hemoconcentration

54
Q

what is the most critical error a phlebotomist can make?

A

ID error

55
Q

what indicator lets you know you are in the vein when performing a syringe draw?

A

flash of blood

56
Q

what is the longest amount of time a tourniquet should be tied on a patient’s arm prior to venipuncture?

A

less then one minute

57
Q

the alcohol prep pad used for cleaning a puncture site is what concentration?

A

70% isopropyl alcohol

58
Q

give a couple examples of coagulation test

A

PT
PTT
fibrinogen
D-Dimer

59
Q

what color tube is coagulation tests collected in?

A

blue

60
Q

what is the anticoagulant used in the blue tubes?

A

sodium citrate

61
Q

what should you do if your patient does not have an ID band on but is not conscious or able to ID themselves?

A

have a nurse put an ID band on

62
Q

how should you handle questions from a patient about what tests you’re drawing and what they mean?

A

be as brief as possible and refer them to their doctor

63
Q

do you ever draw a specimen from above an IV? why?

A

no because of the dilution that will occur in the sample

64
Q

what should you do if you cannot draw on a patient’s arms because of multiple IV’s?

A

ask nurse to stop the IV wait 10 minutes then draw

65
Q

why is it so important to mix anticoagulant tubes thoroughly after filling them?

A

mix equally with additives because micro-clots may occur

66
Q

what is the smallest bore on a needle?

A

25

67
Q

what is the largest bore on a needle?

A

18

68
Q

small veins on a patient may collapse when what occurs?

A

blood drawn to quickly

69
Q

what indication might alert you to the possibility that you have entered an artery during venipuncture?

A

bright red blood pulsing into the tube

70
Q

what is the number one cause of hemoconcentration during venipuncture/

A

long tourniquet application

71
Q

is water considered an acceptable thing to drink before a fasting sample is drawn?

A

yes

72
Q

is coffee acceptable to drink before drawing of fasting sample?

A

no

73
Q

is smoking acceptable before fasting sample?

A

no

74
Q

normal serum appearance

A

clear straw color

75
Q

normal plasma appearance

A

slightly opaque straw color

76
Q

hemolyzed serum appearance

A

clear red

77
Q

icteric serum appearance

A

brownish-yellow

78
Q

lipemic serum appearance

A

milky-white

79
Q

color tube of heparin

A

green

80
Q

color tube of no additive

A

red

81
Q

color tube of EDTA

A

lavender

82
Q

color tube of sodium citrate

A

blue

83
Q

color of tube of silicon serum separation material

A

red/gray/gold

84
Q

color of tube sodium fluoride

A

gray

85
Q

color of tube SPS

A

yellow