3.3.13 Amino acids, proteins + DNA Flashcards
(6 cards)
1
Q
how can we identify amino acids?
A
- fluorescent dyes + UV light
- iodine/ninhydrin solution
2
Q
how to calculate rf value of amino acid
A
distance travelled by spot / distance travelled by solvent
3
Q
what affects the rf value
A
- temperature
- makeup of the TLC plate
- solvent
4
Q
what are the conditions for hydrolysis (protein -> amino acid)
A
- 6 moldm-3 HCl
- 110 degrees celsius
- reflux for 24 hours
5
Q
describe the primary protein structure
A
shows the individual sequence of amino acids that make up the protein
6
Q
A
