Section 13: Cell Growth And Metabolism Flashcards
Log phase
2nd phase
Cells start to multiply by doubling every 30 minutes
What are the phases of bacterial growth?
Lag phase
Log phase
Stationary phase
Death phase
Lag phase
1st phase
Cells adapt to rich supply of nutrients in prep for growth
Stationary phase
3rd phase
Cells are dividing and dying at the same rate due to limited nutrients and accumulation of waste products
Death phase
4th phase
Don’t receive sufficient energy or nutrients to live
72 hours or more after inoculation
How do you determine bacterial cell numbers?
Perform serial dilutions at each time point, spread aliquots of resulting dilutions on Petri dish, incubate, and count how many colonies grow
Turbidity
A way of measuring the number of cells
How cloudy the liquid culture is
Catabolism
The breakdown of complex materials into building blocks or energy the cell can use for synthesis
Anabolism
The use of energy and building blocks to produce new cellular materials, such as macromolecules
Metabolism
All the chemical processes that occur in a cell
Enzymes as catalyst for energy
Decrease the amount of heat or activation energy required to link 2 molecules together
glucose + fructose + ATP ➡️ sucrose + H2O + ADP + Pi
Glycolysis
Breakdown of sugar
Krebs cycle
Uses oxygen to degrade the carbon completely to carbon dioxide, water, ATP, and energy containing electrons that are passed on to the electron transport chain
Electron transport chain
Carriers use the electron derived energy to pump protons out of the sale
Fermentation
The incomplete catabolism of sugars to produce alcohol
