DNA Extraction Flashcards
DNA polymerase
Enzyme that assembles nucleotides to make more DNA
DNA fingerprinting
Uses patterns of sliced and sorted DNA to identify a person
Polymerase Chain Reaction
Quickly makes many copies of DNA without using cells
DNA Primer
Small piece of DNA that allows DNA polymerase to attach and begin copying
DNA Cloning
Reprograms bacteria to make many copies of a gene we want
Restriction enzyme
Cuts DNA at set locations based on the nucleotide sequence
Recombinant DNA
DNA blended from multiple sources
DNA Ligase
enzyme that “glues” DNA fragments together
Plasmid
Extra loop of bacterial dNA
Sticky ends
Unattached nucleotides at ends of dNA strands after restiction enzymes make zigzag cuts
Gel Electrophoresis
separates DNA fragments by size
Nucleic Acid Probe
Identifies specific DNA segments using radioactive;fluorescent tags
Arthrobacter luteus
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Haemophilus influenzae
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Bacillus amyloli
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