Genes Flashcards

1
Q

Where are genes located?

A

On the chromosomes.

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2
Q

Are DNA control sequences always the same?

A

No, they are protein and tissue specific.

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3
Q

What is an exon?

A

A coding portion of DNA.

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4
Q

What is an intron?

A

A non-coding portion of DNA.

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5
Q

What comprises a gene?

A

Both exons and introns.

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6
Q

Is the whole gene transcribed?

A

Yes, but introns are removed before translation.

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7
Q

Do both eukaryotes and prokaryotes have introns?

A

No, only eukaryotes have introns.

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8
Q

Why don’t prokaryotes have introns? Give 2 reasons.

A
  1. Smaller genomes = faster replication.

2. Less material to transcribe/translate conserves energy.

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9
Q

Do mitochondria have their own DNA?

A

Yes.

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10
Q

What are mitochondria evidence of?

A

Endosymbiosis, they were previously free-living alpha-proteobacteria.

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11
Q

How many mitochondria does each cell contain?

A

200-100,000.

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12
Q

Is mtDNA associated with proteins like nuclear DNA?

A

No.

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13
Q

Is mtDNA linear like nuclear DNA?

A

No, it is circular.

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14
Q

Does mtDNA contain introns?

A

No (or v. little).

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15
Q

Is the mutation rate of mtDNA higher or lower than that or nuclear DNA?

A

Higher.

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16
Q

What kind of inheritance does mtDNA follow?

A

Matrilineal.

17
Q

There are natural events that can change DNA sequences. List 5.

A
  1. Meiosis (sex)
  2. Bacterial or viral infection
  3. Transposable elements
  4. DNA uptake, e.g. bacterial conjugation
  5. Mutation
18
Q

Define genetic engineering.

A

“Creating and exploiting novel DNA sequences outside the range that can be achieved naturally.”

19
Q

How is recombinant DNA achieved?

A
  1. Isolate particular gene
  2. Amplify
  3. Manipulate
  4. Transform host
20
Q

What are the 6 major applications of DNA technology?

A
  1. Protein production
  2. GM crops/livestock
  3. Organ transplants
  4. Gene therapy
  5. Disease diagnosis
  6. DNA profiling/forensics