Microbiology basics

Question 1

name the gram positive cocci

Answer 1

staph, strep

Question 2

name the gram positive rods

Answer 2

CCBLMG:
clostridium
corynebacterium
bacillus
listeria
mycobacteria
gardnerella

Question 3

name the 13 enterics (subset of gram negative rods)

Answer 3

E. coli
shigella
salmonella
yersinia
klebsiella
proteus
enterobacter
serratia
vibrio
campylobacter
helicobacter
pseudomonas
bacteroides

Question 4

name the 3 respiratory gram negative rods

Answer 4

haemophilus
legionella
bordetella

Question 5

name the 4 zoonotic gram negative rods

Answer 5

francisella
brucella
pasteurella
bartonella

Question 6

name the 2 filamentous gram positives

Answer 6

actinomyces

nocardia

Question 7

name the 2 pleomorphic gram negatives

Answer 7

rickettsia

chlamydia

Question 8

name the 3 gram positive spirochetes

Answer 8

treponema
borrelia
leptospira

Question 9

name the microbe with no cell wall

Answer 9

mycoplasma

Question 10

name the 6 microbes that don’t gram stain well

Answer 10

These Microbes May Lack Real Color
treponema
mycoplasma
mycobacteria
legionella
rickettsia
chlamydia

Question 11

name 5 microbes that require Giemsa stain

Answer 11

Certain Bugs Really Try my Patience
chlamydia
borrelia
rickettsia
trypanosomes
plasmodium

Question 12

what does PAS stain and what is it used for?

Answer 12

glycogen, Whipple disease

Question 13

when do we use Ziehl-Neelson stain?

Answer 13

acid fast organisms (nocardia, mycobacterium)

Question 14

when do we use india ink?

Answer 14

cryptococcus neoformans

Question 15

when do we use silver stain?

Answer 15

fungi
legionella
helicobacter

Question 16

H. influenza needs:

Answer 16

chocolate agar with factors V (NAD+) and X (hematin)

Question 17

N. gonorrhea and meningitidis need:

Answer 17

Thayer-Martin (VPN)
Vancomycin
Polymyxin
Nystatin

Question 18

B. pertussis needs:

Answer 18

Bordet-Gengou potato agar

Question 19

C. diphtheria needs:

Answer 19

tellurite agar, Loffler medium

Question 20

M. tuberculosis needs:

Answer 20

Lowenstein-Jensen agar

Question 21

M. pneumonia needs:

Answer 21

Eaton agar

Question 22

lactose fermenting enterics need:

Answer 22

pink colonies on MacConkey agar

Question 23

legionella needs:

Answer 23

charcoal yeast extract agar

Question 24

fungi need:

Answer 24

sabouraud agar

Question 25

name the 4 obligate aerobes

Answer 25

Nagging Pests Must-Breathe
nocardia
pseudomonas
mycobacterium

Question 26

name the 3 obligate anaerobes

Answer 26

clostridium
bacteroides
actinomyces

Question 27

name the two obligate intracellular microbes

Answer 27

rickettsia

chlamydia

Question 28

name 8 facultative intracellular microbes

Answer 28

Some Nasty Bugs May Live FacultativeLY
salmonella
neisseria
brucella
mycobacterium
listeria
francisella
legionella
yersinia pestis

Question 29

microbes with capsules

Answer 29

SHiNE SKiS

Strep pneumonia
Haemophilus influenza
Neissera meningitidis 
E. coli
Salmonella
Klebsiella
Strep (group B)

Question 30

catalase positive microbes

Answer 30

you need PLACESS for your cats

pseudomonas
listeria
aspergillus
candida
E. coli
S. aureus
Serratia

Question 31

urease positive microbes

Answer 31

CHuck norris hates PUNKSS
cryptococcus
H. pylori
Proteus
Ureaplasma
Nocardia
Klebsiella
S. epidermidis
S. saprophyticus