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Sero by: (FB: 28spud) Follow thx > 3S LAB: Rapid tests pt. 2 > Flashcards

3S LAB: Rapid tests pt. 2 Flashcards

(69 cards)

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1
Q

Time required for HBsAg results

A

15 minutes

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2
Q

Analyte of interest in HBsAg test

A

HBsAg

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3
Q

The test line of HBsAg rapid test contains:

i. Mouse monoclonal anti-HBs antibody
ii. Goat anti-human Ig
iii. Goat anti-HBV Ig
iv. Recombinant HBV antigen

A

i

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4
Q

The control line of HBsAg rapid test contains:

i. Mouse monoclonal anti-HBs antibody
ii. Goat anti-human Ig
iii. Goat anti-HBV Ig
iv. Recombinant HBV antigen

A

ii

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5
Q

How much diluent is required in HCV rapid test?

A

4 drops

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6
Q

Time required for HCV rapid test results

A

5-20 minutes

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7
Q

Analyte of interest for HCV rapid test

A

Anti-HCV

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8
Q

The test line of HCV rapid test contains:

i. Mouse monoclonal anti-HCV antibody
ii. Goat anti-human Ig
iii. Goat anti-HCV Ig
iv. Recombinant HCV capture antigen

A

iv

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9
Q

The control line of HCV rapid test contains:

i. Mouse monoclonal anti-HCV antibody
ii. Goat anti-human Ig
iii. Goat anti-HCV Ig
iv. Recombinant HCV capture antigen

A

ii

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10
Q

How much diluent is required in the HBsAg rapid test?

A

None required

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11
Q

Confirmatory test for hepatitis C virus

A

Nucleic Acid Test (NAT)

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12
Q

In HCV rapid test, how much sample is used?

A

10 uL plasma/serum
20 uL whole blood

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13
Q

How much diluent is required for HIV rapid test?

A

4 drops

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14
Q

In HIV rapid test, how much sample is used?

A

10 uL plasma/serum
20 uL whole blood

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15
Q

How much time is required for HIV rapid test result?

A

10-20 minutes

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16
Q

Analyte of interest in HIV rapid test

A

IgG, IgM, IgA anti-HIV-1 and HIV-2

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17
Q

The test line 1 of HIV rapid test contains:

i. Mouse monoclonal anti-HIV antibody
ii. Goat anti-human Ig
iii. Recombinant HIV antigen (gp41, gp24)
iv. Recombinant HIV antigen (gp36)

A

iii

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18
Q

The test line 2 of HIV rapid test contains:

i. Mouse monoclonal anti-HIV antibody
ii. Goat anti-human Ig
iii. Recombinant HIV antigen (gp41, gp24)
iv. Recombinant HIV antigen (gp36)

A

iv

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19
Q

In HAV rapid test, how much sample is used?

A

1 drop plasma/serum
2 drops whole blood

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20
Q

How much diluent is required in HAV rapid test?

A

2 drops

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21
Q

How much time is required for the HAV rapid test results?

A

15 minutes

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22
Q

Analyte of interest for HAV rapid test

A

IgG and IgM Abs to HAV

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23
Q

The test line of HAV rapid test contains:

i. Mouse monoclonal anti-HAV antibody
ii. Mouse anti-human Ig-coated particles
iii. Goat anti-HAV Ig
iv. Recombinant HAV antigen

A

ii

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24
Q

Specimen used for COVID-19 rapid test

A

Nasopharyngeal swab

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25
How much diluent is required in COVID-19 rapid test?
5 drops
26
How much time is required for the COVID-19 rapid test results?
15 minutes
27
Analyte of interest for COVID-19 rapid test
SARS-CoV-2 Antigen
28
The test line of COVID-19 rapid test contains: i. Mouse monoclonal anti-SARS-CoV-2 antibody ii. Goat anti-human Ig iii. Goat anti-SARS-CoV-2 Ig iv. Recombinant SARS-CoV-2 capture antigen
i
29
The control line of COVID-19 rapid test contains: i. Mouse monoclonal anti-SARS-CoV-2 antibody ii. Mouse monoclonal anti-chicken IgY iii. Goat anti-SARS-CoV-2 Ig iv. Recombinant SARS-CoV-2 capture antigen
ii
30
How much diluent is required for the dengue duo test?
3 drops
31
The test line of Dengue Duo rapid test contains: i. Mouse monoclonal anti-NS-1 antibody ii. Mouse monoclonal anti-chicken IgY iii. Goat anti-NS-1 Ig iv. Recombinant NS-1 capture antigen
i
32
The test line of Dengue Duo rapid test contains: i. Mouse monoclonal anti-NS-1 antibody ii. Mouse monoclonal anti-chicken IgY iii. Goat anti-NS-1 Ig iv. Recombinant NS-1 capture antigen
ii
33
Analyte of interest for the Dengue Duo Test
IgG and IgM (for dengue duo) Anti-Dengue NS1 (for NS1)
34
How much diluent is required for Chikungunya rapid test?
2 drops
35
How much time is required for the dengue duo test results?
15-20 minutes
36
How much time is required for Chikungunya rapid test?
15-20 minutes
37
Analyte of interest for Chikungunya rapid test
Chikungunya IgG and IgM antibodies
38
How much sample is used in Malaria rapid test?
5 uL whole blood
39
How much diluent is required in Malaria rapid test?
4 drops
40
Time required for Malaria rapid test results
15-30 minutes
41
Analyte of interest for Malaria rapid test
Malaria Antigen
42
The P.f. region of the test line of Malaria rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. Mouse monoclonal anti-pLDH iii. Mouse monoclonal anti-HRP2 iv. Recombinant pPM-LDH antigen
iii
43
The pan region of the test line of Malaria rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. Mouse monoclonal anti-pLDH iii. Mouse monoclonal anti-HRP2 iv. Recombinant pPM-LDH antigen
ii
44
The control line of Malaria rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. Mouse monoclonal anti-pLDH iii. Mouse monoclonal anti-HRP2 iv. Recombinant pPM-LDH antigen
iv
45
T/F: The malaria rapid test can differentiate P. falciparum from other Plasmodium species
True
46
How much specimen is used for Leptospira IgM rapid test?
10 uL serum/plasma
47
How much diluent is required for the Leptospira rapid test?
3 drops
48
How much time is required for the leptospira rapid test results?
15-20 minutes
49
Analyte of interest for leptospira rapid test
Anti-Leptospira IgM and IgG
50
The test line of Leptospira rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. Anti-Leptospira IgM iii. Monoclonal anti-Leptospira-env-gold iv. Monoclonal anti-human IgM and IgG
iii
51
The control line of Leptospira rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. Anti-Leptospira IgM iii. Monoclonal anti-Leptospira-env-gold iv. Monoclonal anti-human IgM and IgG
iv
52
How much specimen is used in Leptocheck?
10 uL serum/plasma
53
How much diluent is used in Leptocheck?
5 drops
54
How much time is required for the Leptocheck results?
15-30 minutes
55
Analyte of interest for Leptocheck
IgM antibodies
56
The control line of Leptospira rapid test contains: i. Agglutinating sera for rabbit globulin ii. Anti-Leptospira IgM iii. Monoclonal anti-Leptospira-env-gold iv. Monoclonal anti-human IgM and IgG
i
57
Positive results for Leptocheck are confirmed by ___
Microscopic Agglutination Test (MAT)
58
How much sample is required for H. pylori rapid test?
10 uL serum/plasma
59
How much diluent is required in H. pylori rapid test?
3 drops
60
How much time is required for H. pylori rapid test results?
10-20 minutes
61
Analyte of interest for H. pylori rapid test
H. pylori IgM, IgA
62
The test line of H. pylori rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. H. pylori capture antigen iii. Goat anti-H. pylori polyclonal antibody iv. Monoclonal anti-human IgM and IgG
ii
63
The test line of H. pylori rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. H. pylori capture antigen iii. Goat anti-H. pylori polyclonal antibody iv. Monoclonal anti-human IgM and IgG
iii
64
How much sample is used in S. typhi rapid test?
1 drop whole blood
65
How much diluent is used in S. typhi rapid test?
2 drops
66
How much time is required for S. typhi rapid test results?
15-20 minutes
67
Analyte of interest for S. typhi rapid test
S. typhi IgM or IgG
68
The test line of S. typhi rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. S. typhi capture antigen iii. Goat anti-S. typhi polyclonal antibody iv. Mouse anti-human IgM and IgG
iv
69
The control line of S. typhi rapid test contains: i. Mouse monoclonal anti-chicken IgY ii. S. typhi capture antigen iii. Goat anti-S. typhi polyclonal antibody iv. Mouse anti-human IgM and IgG
iii

Sero by: (FB: 28spud) Follow thx (28 decks)

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