3S [LEC]: Blood Component Preparation and Therapy

Question 1

What will be the product/s if whole blood undergoes light spin?

Answer 1

Platelet rich plasma
Packed RBC

Question 2

What will be the product/s if whole blood undergoes heavy spin?

Answer 2

Packed RBC
Platelet poor plasma

Question 3

If platelet poor plasma is frozen, within 8 hours it becomes ___

Answer 3

Fresh frozen plasma

Question 4

If platelet rich plasma undergoes heavy spin, what will be the product/s?

Answer 4

Platelet concentrate
Cryo poor plasma

Question 5

Allow blood components to flow from 1 bag to another via the integrated tubing system

Answer 5

Plasma expressor

Question 6

Allow 2 separate blood bags to be connected via their PVC tubing without breaching the integrity of either container

Answer 6

Sterile connection device

Question 7

Temperature setting of plasma freezers

Answer 7

-18C

Question 8

Storage temperature requirement of whole blood

Answer 8

1-6C

Question 9

Transport requirement of whole blood

Answer 9

1-10C

Question 10

The hct and hgb increase after whole blood transfusion is seen after ___

Answer 10

24-48 hours

Question 11

Storage temperature requirement of packed RBCs

Answer 11

1-6C

Question 12

In packed RBCs, hct should not exceed ___

Answer 12

80%

Question 13

Indicated for patients needing to restore oxygen carrying capacity

Answer 13

Packed RBCs

Question 14

Indicated in patients with anti-WBC antibody

Answer 14

Leukopoor/ Leukoreduced RBCs

Question 15

Storage temperature requirement of leukopoor/ leukoreduced RBCs

Answer 15

1-6C

Question 16

In leukopoor/ leukoreduced RBCs, the hct should be at least ___

Answer 16

85%

Question 17

In leukopoor/ leukoreduced RBCs, the WBCs should be at least ___

Answer 17

<5 x 10^6

Question 18

Reduces the chances of having FNHTR or TRALI

Answer 18

Leukopoor/ Leukoreduced RBCs

Question 19

The only FDA-approved rejuvination solution

Answer 19

Rejuvesol

Question 20

Shelf life of rejuvenated RBCs

Answer 20

24 hours after washing

Question 21

Indicated in reducing chances of graft versus host disease

Answer 21

Irradiated RBCs

Question 22

Rejuvenated RBCs can be prepared ___ days after expiration date

Answer 22

3 days

Question 23

Storage temperature requirement of rejuvenated RBCs

Answer 23

1-6C

Question 24

Indicated if the patient has plasma allergy or if the donor is positive for antibody screening

Answer 24

Washed RBCs

Question 25

Indicated in patients with anti-IgA antibody

Answer 25

Washed RBCs

Question 26

Washed RBCs should have hct of ___

Answer 26

70-80%

Question 27

Shelf life of washed RBCs

Answer 27

24 hours

Question 28

Has the longest shelf life

Answer 28

Frozen RBCs

Question 29

Shelf life of frozen RBCs

Answer 29

10 years

Question 30

Storage temperature requirement of frozen RBCs in high glycerol method

Answer 30

-65C

Question 31

Storage temperature requirement of frozen RBCs in low glycerol method

Answer 31

-120C

Question 32

Storage temperature requirement of frozen RBCs in agglomeration method

Answer 32

-65C

Question 33

Cryoprotective agent that penetrates RBC membrane and used in preserving frozen RBCs

Answer 33

Glycerol

Question 34

Cryoprotective agent that does not penetrate RBC membrane and instead protects through covering the red cells; used in preserving frozen RBCs

Answer 34

Hydroxyethyl starch (HES)

Question 35

Freezing temperature in frozen RBCs using high glycerol method

Answer 35

-80C

Question 36

Freezing temperature in frozen RBCs using low glycerol method

Answer 36

-196C

Question 37

Freezing temperature in frozen RBCs using high agglomeration method

Answer 37

-80C

Question 38

Shelf life of deglycerolized RBCs

Answer 38

24 hours

Question 39

Deglycerolized RBCs should recover ___% of RBC

Answer 39

80%

Question 40

In deglycerolized RBCs, glycerol should be at ___%

Answer 40

<1%

Question 41

Storage temperature requirement of deglycerolized RBCs

Answer 41

1-6C

Question 42

Shelf life of platelet concentrate

Answer 42

5 days

Question 43

Storage temperature requirement of platelet concentrate

Answer 43

20-24C with constant agitation

Question 44

Platelet concentrate should have atleast ___ platelets

Answer 44

5.5 x 10^10 (random donor) 3 x 10^11 (single donor)

Question 45

Platelet concentrate should have a pH level of ___

Answer 45

>/= 6.2

Question 46

Immediate effect of platelet concentrate transfusion

Answer 46

5 000 - 10 000 plt increase (random) 20 000 - 60 000 plt increase (single donor)

Question 47

Storage temperature requirement of fresh frozen plasma

Answer 47

-18C or lower

Question 48

Storage temperature requirement of thawed fresh frozen plasma

Answer 48

1-6C; 37C

Question 49

Contains maximum levels of both stable and labile coagulation factors

Answer 49

Fresh frozen plasma

Question 50

Shelf life of fresh frozen plasma

Answer 50

1 year

Question 51

Shelf life of thawed fresh frozen plasma

Answer 51

24 hours

Question 52

Immediate effect of fresh frozen plasma

Answer 52

Increased coagulation factors by 20-30%

Question 53

Indicated in hemophilia A and von Willebrand's disease patients

Answer 53

Cryoprecipitate

Question 54

Coagulation factors present in cryoprecipitate

Answer 54

VIII, Fibrinogen, XIII

Question 55

Storage temperature requirement of cryoprecipitate

Answer 55

-18C or colder

Question 56

Shelf life of cryoprecipitate

Answer 56

1 year

Question 57

Shelf life of thawed cryoprecipitate

Answer 57

6 hours

Question 58

Shelf life of pooled cryoprecipitate

Answer 58

4 hours

Question 59

Immediate effect of cryoprecipitate

Answer 59

Increase fibrinogen by 5-10 mg/dL

Question 60

Storage temperature requirement of granulocyte concentrate

Answer 60

20-24C without agitation

Question 61

Granulocyte concentrate should have ___ WBCs

Answer 61

1 x 10^10

Question 62

Shelf life of granulocyte concentrate

Answer 62

24 hours

Question 63

Immediate effect of granulocyte concentrate

Answer 63

1-2 x 10^10 per infusion of 4 daily doses