4 Kinetics of Substrate Utilization, Product Formation, & Biomass Production In Cell Cultures Flashcards
Cellular representations are multicomponent
Structured
Single component representations
Unstructured
Considers discrete, heterogeneous individual cells
Segregated
Considers average cellular properties
Unsegregated
All cellular components must be produced in production to their abundances
Balanced growth
Generally, ________, ________, and empirical modesls are used to describe cell growth rate.
Unsegregated & unstructured
It is difficult to obtain useful kinetic information on microbial cell growth from non-uniform condition reactors. Hence it is desirable to study kinetics in reactors that are ideal.
True
[Ideal Batch Reactor]
To get the “growth” or accumulation of any component i (product biomass) in the reactor:
d/dt (culture volume) (mole conc.of component i)
d/dt (V) (Ci) = V (ri)
If no liquid is added to or removed from the reactor and if gas stripping of
culture liquid is negligible
d (ci)/ dt = ri
Direct measurements of the change in conc. of the component i gives us the rate of its formation
[Ideal CSTR]
Steady state conditions:
Rate of addition to reactor - Rate of removal from reactor + Rate of formation within the reactor = 0
Reciprocal of mean holding/residence time
Dilution rate, D
Property of CSTR
- Kinetics is more straightforward
- Can adjust to steady environment &achieve or closely approx a state of balanced growth
u (myu)
specific cell growth rate of the cells (1/time)
If xi=0, the liquid stream feed consist of sterile nutrients.
x cannot be determined
Dxi = (D-u) x
Nonzero cell population can be maintained when D=u
True
Kinetic models for growth cells can be done knowing substrates can be….
growth-limiting
Functional relationship between. The specific growth rate, u, & essential compound’s conc..
Monod Growth Kinetics
Who introduced classification of product formation kinetics for Batch Reactor Fermentation?
F.H. Deindoerfer
[Classification of Fermentation Patterns]
Nutrients converted to products in a fixed stoichiometry without accumulation of intermediates
Simple
[Classification of Fermentation Patterns for Batch Reactor]
Nutrients converted to products in variable stoichiometry proportion without accumulation of intermediates
Simultaneous
[Classification of Fermentation Patterns for Batch Reactor]
Nutrients converted to product with accumulation of an intermediate
Consecutive
[Classification of Fermentation Patterns for Batch Reactor]
Nutrients completely converted to intermediate before conversion to product, or selectively converted to product in preferential order.
Stepwise
