Block 2 Glycolysis

Question 1

GLUT-1

Answer 1

ubiquitous, highest in erythrocytes

Question 2

GLUT-2

Answer 2

liver, beta cells of pancreas, small intestine high KM

Question 3

GLUT- 3

Answer 3

neurons, placenta, testes, high affinity, low km

Question 4

GLUT-4

Answer 4

muscle and adipose tissue

Question 5

GLUT-5

Answer 5

highest in small intestine

Question 6

GLUT-4 regulation

Answer 6

insulin triggers exocytosis of GLUT-4 which increases uptake of glucose by 20 fold

Question 7

GLUT-2 significance

Answer 7

important to keep blood glucose levels regular in fed and fasted state

Question 8

Glycolysis

Answer 8

10 step converting gulcose into 2 pyruvate, genertaing ATP and NADH

Question 9

Rxn 1-5

Answer 9

energy consumption

Question 10

rxn 6-10

Answer 10

Energy production

Question 11

RXN 1

Answer 11

Hexokinase/Gluckinase
Glucose + ATP -> Glucose 6-phosphate + ADP
traps glucose in the cytosol of the cell

Question 12

RXN 2

Answer 12

Phophoglucoisomerase
shifting the carbonyl carbon from C1 to C2
Glucose 6 phosphate -> fructose 6 phosphate

Question 13

RXN 3

Answer 13

Committed step! PFK-1

fructose 6-phosphate + ATP -> Fructose 1,6 biphosphate

Question 14

RXN 4

Answer 14

Aldolase

Fructose 1,6 bisphospate -> dihydroxyacetone phosphate (ketose) + glyceraldehyde 3 phosphate (aldose)

Question 15

RXN 5

Answer 15

Triosphosphate isomerase

Dihydroxyacetone phosphate -> glyceraldehyde 3 Phosphate (GAP)

Question 16

RXN 6

Answer 16

GAPDH

glyceraldehyde 3- phosphate + NAD -> 1,3 BPG + NADH

Question 17

RXN 7

Answer 17

Phosphoglycerate Kinase

1,3 BPG + ADP -> 3-Phosphoglycerate + ATP

Question 18

RXN 8

Answer 18

Phosphoglycerate Mutase

Shifting phosphate from C3 to C2 (product is 2-phosphoglycerate)

Question 19

RXN 9

Answer 19

enolase

2-phosphoglycerate -> phosphoenolpyruvate + h2o

Question 20

RXN 10

Answer 20

pyruvate kinase

phosphoenolpyruvate + ADP -> pyruvate + ATP

Question 21

Negative regulators of PFK 1

Answer 21

ATP, citrate, Phosphenol pyruvate

Question 22

Positive regulators of PFK 1

Answer 22

AMP, Fructose 6-phosphate, Fructose 1,6-biphosphate, fructose 2,6-biphosphate

Question 23

Enzymes that use ATP

Answer 23

PFK-1 Hexokinase/Glucokinase

Question 24

Enzymes that make High energy substrates

Answer 24

GADPH, Enolase

Question 25

Produces NADH

Answer 25

GAPDH

Question 26

Enzymes that facilitate Substrate level phophorylation

Answer 26

phophoglycerate kinase, pyruvate kinase