ch 12 Flashcards
planer chromatography inc column chromatography inc resolution is measure of chromatographic separation incomplete than Rs is \_\_\_\_\_\_ baseline is greater than \_\_\_\_\_
paper and thin layer
gas and liquid
less than .8
greater than 1.25
ion exhange chromotography partition absorption size exclsion affinity
seperated amino acids, glycated hemobgloin and removes ions form aqueous solutions–> desperation based of ions
partition: separation based on solute partitioning between liquid phase
absorption–> based on adsorption/desorption steps –> used in volatile gases
size exclusion: separate based on size –> protien and nucleic acids
affinity–> immobilized in stationary phase
immunoaffinity–> pregnancy test, rapid microbial antigen
thin layer chromotography describe Rf what is the applications automated vs manual gas chromotograph describe carrier gas liquid chromotography
distance from application point to solute center/ distance from application point to mobile phase
application in id of drugs in urine, anayis of AA in urine and lipids
manuel: less quantitiave more labor and technique dependent
tlc adv. less cost
gas chromatograph gas mobile phase carry volatile gas through
carrier gas nitrogen helium and argon
liquid separation based on differential distribution of solute between a liquid mobile and stationary phase
chromatography how do they separate
solutes w/ greater affinity for stationary phase reside in the ____ and migrate ____
less affinity reside in ___ an move ____
seperate as a result of thier differential distribution between stationary and mobile phase
stationary phase and migrate slowly
mobile phase and move faster
