4.3 Flashcards
(18 cards)
Central Dogma…
DNA–>RNA–>Proteins
DNA to RNA
Transcription
RNA to Proteins
Translation
Transcribed DNA strand…
Template Strand
Untranscribed DNA strand…
coding strand
What is the main enzyme in transcription of DNA to RNA?
RNA Polymerase
The coding strand of this templete stand…
ATGCCGATG
TACGGCTAC
The mRNA of this templete strand…
ATGCCGATG
UACGGCUAC
What is the anatomy of a basic eukaryotic gene?
Promoters, Enhancers, Exons, and Introns.
Start site of transcription
Promoter
Additional binding site for transcription factors
Act as an on/off switch
Enhancer
Sequenced that will be present in mRNA
Exons
Will be spliced out and removed
Introns
Which stage?
Transcription factors bind to promoter regions
Stage 1: Initiation
Which Stage?
RNA polymerase breaks free of the Transcription factors
A protective 5’ cap is added
Stage 2: Elongation
Which Stage?
At the polyaderylaition site, the termination sequenced is recognized and cut.
Stage 3: Termination
What happens during the splicing process?
Edit out the introns
How can one gene code for multiple proteins?
The different combinations of exons