4th

Question 1

Initiation of DNA replication in eukaryotic cells begins with what

Answer 1

The binding of the origin recognition complex (ORC)

Question 2

ORC is consist of

Answer 2

Multi subunits DNA binding complex (6 subunits) in a atp dependent manner

Question 3

pre-RC formation involves the ordered assembly of many replication factors including:

Answer 3

1.ORC
2.Cdc6(with ATP)
3.Cdt1+Mcm2-7

Question 4

Why is the pre-rc assembly during G1 is required

Answer 4

For replication license

Question 5

What happens when atp hydrolysis of cdc6 protien

Answer 5

Loading of the head to head dimer of Mcm2-7 on the DNA strand

Question 6

When does the pre-rc complex convert into the initiation complex

Answer 6

During cell cycle transition from g1 to S

Question 7

Converting pre-rc complex into initiation complex happens by the action of what

Answer 7

CDK+DDK

Question 8

DDK targets what

Answer 8

Loaded helicase

Question 9

CDK target what

Answer 9

Two other replication protiens»phosphorylation&raquo_space;Cdc45 & GINS protiens&raquo_space;bind to Mcm2-7 helicase

Question 10

What is the active form of Mcm2-7dna helicase

Answer 10

Cdc45-Mcm2-7-gins(CMG)

Question 11

DNA polymerase 3 features

Answer 11

1.Synthesis only in the 5’-3’ direction
2. Cannot start without the presence of rna primers

Question 12

What are Primers

Answer 12

Short RNA molecules, synthesized in the 5’-3’ direction by primase (rna polymerase)