5-PRETRANSFUSION LAB

Question 1

Which test uses reagent red blood cells in hypotonic buffered saline combined with recipient serum or plasma to detect antigen-antibody interaction

Answer 1

Antibody screen

Question 2

Which temperature is used for incubation in antibody screen gel technology

Answer 2

37 degrees Celsius

Question 3

Which reagent in the antibody screen gel card contains polyspecific AHG

Answer 3

LISS/Coombs

Question 4

Which step involves identifying microtubes with donor or patient information in antibody screen

Answer 4

Identify microtubes

Question 5

Which step involves removing aluminum foil from microtubes in antibody screen

Answer 5

Remove aluminum foil

Question 6

Which volume of test cell reagent is pipetted into microtubes in antibody screen

Answer 6

50 microliters

Question 7

Which volume of donor or patient plasma or serum is added to microtubes in antibody screen

Answer 7

25 microliters

Question 8

Which incubation time is used in antibody screen gel technology

Answer 8

15 minutes

Question 9

Which centrifugation time is used in antibody screen gel technology

Answer 9

10 minutes

Question 10

Which result in antibody screen shows agglutinated cells forming a red line or dispersed agglutinates

Answer 10

Positive

Question 11

Which result in antibody screen shows compact button of cells at the bottom of microtube

Answer 11

Negative

Question 12

Which result in antibody screen shows a layer of positive cells at the top and button of negative cells

Answer 12

Mixed field

Question 13

Which test is the final check of ABO compatibility between donor and recipient

Answer 13

Crossmatching

Question 14

Which test detects antibodies in patient serum that react with donor red blood cells not detected in antibody screening

Answer 14

Crossmatching

Question 15

What does a serologic crossmatch consist of

Answer 15

Mixing recipient plasma with donor red cells

Question 16

What does a nonreactive serologic crossmatch indicate

Answer 16

Donor unit is compatible and safe for transfusion

Question 17

Which phase of crossmatch involves mixing patient serum with donor red cells and immediate centrifugation

Answer 17

Phase I Saline/Immediate Spin

Question 18

Which temperature and incubation time is used in Phase II of crossmatch

Answer 18

37 degrees Celsius for 10 minutes

Question 19

What reagent is added in Phase II of crossmatch

Answer 19

DiaLISS

Question 20

What is done after incubation in Phase II of crossmatch

Answer 20

Centrifuge and examine for hemolysis and agglutination

Question 21

What is the purpose of Phase III Direct Coombs/Anti-Human Globulin phase

Answer 21

Detect antibodies bound to red cells

Question 22

What reagent is added in Phase III of crossmatch

Answer 22

Anti-human globulin serum

Question 23

How many times are cells washed in Phase III of crossmatch

Answer 23

At least 3 times

Question 24

What indicates incompatibility in Phase III of crossmatch

Answer 24

Reactivity or hemolysis at any phase

Question 25

What should be done if test results are visually negative in Phase III

Answer 25

Examine under microscope

Question 26

Who should check test results in crossmatch

Answer 26

Second medical technologist

Question 27

Which test is useful for identifying patients with auto-antibodies

Answer 27

Autocontrol

Question 28

Which condition may make finding a compatible blood unit difficult

Answer 28

Presence of auto-antibodies

Question 29

What is the direct Coombs test used for

Answer 29

Detection of in vivo coating of red blood cells with globulins

Question 30

Which specimen is used in the direct Coombs test

Answer 30

Washed red blood cells from patient or donor

Question 31

Which reagent is used in the direct Coombs test

Answer 31

Antiglobulin reagent

Question 32

Which disease is diagnosed using the direct Coombs test

Answer 32

Hemolytic disease of the newborn

Question 33

Which disease is diagnosed using the direct Coombs test

Answer 33

Autoimmune hemolytic anemia

Question 34

Which investigations are performed using the direct Coombs test

Answer 34

Red blood cell sensitization caused by drugs + Transfusion reactions

Question 35

What are the post-analytical procedures in pretransfusion testing

Answer 35

Labeling of unit with product and patient identification + Generation of compatibility report

Question 36

What information is included on the blood unit label

Answer 36

Product details + Patient identification information

Question 37

Which report accompanies the blood product

Answer 37

Compatibility report

Question 38

When must donor units be labeled

Answer 38

At time of issue

Question 39

What must be confirmed by the transfusionist at bedside

Answer 39

Recipient identification + Donor unit information

Question 40

When can blood components not be issued

Answer 40

Until all discrepancies are resolved

Question 41

What must be inspected before issuing blood components

Answer 41

Hemolysis + Clots

Question 42

What is done if an adverse event related to transfusion occurs

Answer 42

Investigated according to policy