NLS Flashcards
1
Q
NLS
A
Nuclear localization sequence
2
Q
NLS characteristics
A
not necessarily N-terminus; basic; not cleaved; importins and GTPase
3
Q
a,b importins
A
responsible for recognizing NLS and translocating it to the nucleus
4
Q
Ran GTP
A
responsible for removing a,b importins
5
Q
CAS
A
GTPase activity; hydrolyses ran GTP and causes its translocation out of the nucleus
6
Q
NTF2
A
responsible for bringing ran-gdp into the nucleus
7
Q
ran-gef
A
phosphorylates Ran-GDP
8
Q
NLS mode of action
A
1) a,b importins bind to NLS of protein and translocate it into the nucleus 2) Ran-GTP binds to a,b importins and causes their removal 3) CAS hydrolyzes ran GTP causing its translocation out of the nucleus 4) NFT2 binds ran-GDP bringing it back into the nucleus 5) Ran-GEF causes phosphorylation of Ran-GDP
