Biochemisitry Flashcards
What is biochemistry
The study of molecules that make up living organisms. Inorganic molecules occur naturally and organic molecules have to be synthesized by plants
Monomers are organic macromolecules. Monomers of protein, carbs and lipids
Amino acids, glucose, glycerol and 3 fatty acids
Three types of carbs
Monosaccharides- 1sugar- glucose and fructose
Disaccharides- 2sugars- Maltose and sucrose
Polysaccharides- many sugars- starch (glucose in plants) glycogen (glucose in animals) cellulose (plant cell walls)
Fats basic stuff
Carbon hydrogen and oxygen, solid from animals or liquid from plants and fish
Functions if fat molecules
Contain more energy than carbs and protein
Provides structure in cell membranes
Absorbs nutrients (vitamin A + B)
Stores energy as oil
Provides insulation for nerve fibers
Protects organs against shock and movement
Proteins basic stuff
Carbon hydrogen oxygen nitrogen
Consists of 50 or more amino acids joined by peptide bonds
20 amino acids are made by plants
Position of amino acids determines the position of cross-linkage bonds that hold the polypeptide chain together this determines its shape
Protein molecule shapes
Collagen a fibrous protein
Myoglobin a goblular protein
Denatured protein
Exposed to high temperatures and unfavorable pH. Cross linkage bonds will break. Protein will lose its shape and its ability to function
Hemoglobin, insulin, actin and myosin, collagen, keratin, albumin, histones
Red blood cell pigment Controls blood sugar Found in muscle with contractile function Connective tissue hair and nails Egg white Chromosome structure
Starch test
Brown iodine
Blue ur black +
Unchanged -
Glucose test
10 drops Benedicts solution
Hot water bath 2 min
Green, yellow, orange +
Unchanged blue -
Sucrose test
5 drops HCl Hot water bath 10 drops Benedicts Hot water bath Green, yellow, orange + Unchanged blue -
Protein/biuret test
10 drops NaOH
6 drops copper sulfate
Purple or lilac after shake +
Rose pink or black -
Fat test ethanol method
Solid crush with mortar add alcohol filter substance add water milky emulsion
Liquid add alcohol let stand add water milky emulsion
Fat test with ether
Liquid add ether allow to stand for 15 min
Filter on filtrate allow it to evaporate fat stain indicates positive test