Stains

Question 1

What is hematoxylin - pH, bind to what pH molecules, DNA/RNA color, why

Answer 1

basic - binds to acidic molecules - DNA/RNA appear blue/black = dark stain - acid molecules are basophilic

Question 2

what is eosin - pH, color, binds to what molecules

Answer 2

acidic - red dye - basic molecules will bind to eosin - esocinophilic (acidophilic) - proteins stain with these - collagen is very eosinophilic

Question 3

periodic acid Schiff (PAS) dyes

Answer 3

stain sacchrides

Question 4

osmuim/sudan black

Answer 4

fixative = not stain! used to see lipids after H&E

Question 5

can lipids be seen with stains?

Answer 5

No! they are washed out during dehydration with organic solvents

Question 6

Masson’s trichrome

Answer 6

Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei.

Question 7

Methylene blue

Answer 7

basic, blue good for staining specific granules in WBCs (azurophilic)

Question 8

Romanovsky Stain

Answer 8

methylene blue + eosin for blood film prep!