Lecture 2 - DNA Replication and Repair Flashcards
1
Q
How do mutations occur in a broad sense?
A
Errors in DNA replication and repair pathways as well as environmental factors
2
Q
What are important aspects of DNA/protein interactions?
A
Hydrogen-bonding between amino acids and nitrogenous bases, most base contacts are in the major groove, proteins have alpha helices that fit in the major groove, and multiple DNA-binding domains for site-specific recognition
3
Q
Function of DNA polymerase beta
A
Repair DNA synthesis
4
Q
Function of DNA polymerase gamma
A
Mitochondrial DNA synthesis
5
Q
Cytosine spontaneously deaminates to from…
A
Uracil
6
Q
Uracil in DNA is removed by…and this creates an…
A
uracil-DNA glycosidase AP site (apurinic or apyrimidinic)
