Miscellaneous Flashcards

1
Q

Expiration of rejuvenated RBCs in CPD, CPDA-1

A

10 years

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2
Q

Expiration of rejuvenated RBCs in AS-1

A

3 years

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3
Q

Risk of D sensitization after D+ PLT

A

~4%

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4
Q

Preservative for frozen PLT

A

DSMO

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5
Q

PF24RT24 can only be made from …

A

apheresis PLT

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6
Q

Liquid plasma storage temperature and expiration

A

1-6C/5 days after whole blood expiration date

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7
Q

Amount of vWF in unit of cryo

A

80-120 IU

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8
Q

Amount of FXIII in unit of cryo

A

40-60 IU

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9
Q

Albumin and plasma protein fraction composition

A

Albumin: 96% albumin, 4% Igs
PPF: 83% albumin, 17% Igs

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10
Q

DDAVP dose

A

0.3-0.4 ug/kg

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11
Q

cryo dose fo vWD

A

1 unit/10 kg Q 8 hrs

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12
Q

sensitivity of visible hemolysis

A

> 2.5-5 ml of hemolysis

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13
Q

AHTR incidence

A

1/76,000

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14
Q

Fatal AHTR incidence

A

1/1.8 million

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15
Q

PLT contamination rate

A

1/3000

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16
Q

TRALI incidence

A

1/1200 - 190,000

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17
Q

Anaphylactic reaction incidence

A

1/20,000-50,000

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18
Q

Epi dose

A

0.2-0.5 ml of 1:1000 epi (IM/SQ)

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19
Q

pan-positive reactions and auto control at IS/37C but negative at IAT

A

Antibody to reagent

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20
Q

Normovolemic hemodilution storage of blood at RT and 1-6C

A

8 hours and 24 hours

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21
Q

Drug with highest date rate in AIHA

A

Fludarabine

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22
Q

Average RBC engraftment

A

20 days

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23
Q

Average Neutrophil engraftment

A

14 days

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24
Q

Average platelet engraftment

A

19 days

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25
Q

Aedes vector

A

Chikungunya

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26
Q

Anaopheles vector

A

Malaria

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27
Q

Ixodes scapularis

A

Babesia, Lyme disease

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28
Q

Black (Silum) fly

A

Onchocerca

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29
Q

Triatomine

A

Chagas

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30
Q

Bee venom - Apitoxin (melittin)

A

can lead to hemolysis via phospholipase A2

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31
Q

1 PBV removes

A

63% of plasma substance

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32
Q

1.5 PBV removes

A

78% of plasma substance

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33
Q

2 PBV removes

A

85% of plasma substance

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34
Q

2.5 PBV removes

A

92% of plasma substance

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35
Q

3 PBV removes

A

95% of plasma substance

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36
Q

PTP a/w with HLA

A

DRB3*01:01 (DR52)

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37
Q

Cappelini Effect

A

weak D expression when Ce in trans

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38
Q

Cobalt 60 verification

A

Q 6 months

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39
Q

Cesium 137 verification

A

Annually

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40
Q

RBC should be frozen within ___

A

6 days unless rare unit

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41
Q

Mean hgb in apheresis RBC

A

> 60 g/dL with 95% > 50 g/dL

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42
Q

Mean hgb in leukoreduced apheresis RBC

A

> 51 g/dL with 95% > 42.5 g/dL

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43
Q

MKMK phenotype

A

M-N-S-s-U-

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44
Q

En(a) phenotype

A

M-N-

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45
Q

GPB closely a/w with …

A

RhD

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46
Q

Go(a) a/w this partial D type

A

DIV

47
Q

These weak Ds can make allo-anti-D

A

4.2 and 15

48
Q

Matuhasi Ogata phenomenon

A

Nonspecific uptake of IgG by RBCs when another antibody is coating the cells (often seen in eluate)

49
Q

Hubener Thomsen Friedenreich phenomenon

A

“cryptic” antigen uncovered causing polyagglutination (e.g. T antigen)

50
Q

Knull individuals can produce anti-____

A

Ku

51
Q

FVII padua have prolonged PTT with _____ thromboplastin

A

rabbit (normal with bovine)

52
Q

Incidence of bruising and hematoma during donation

A

9-16%

53
Q

Incidence of vasovagal reaction during donation

A

2-5%

54
Q

Incidence of nerve injury during donation

A

0.002%

55
Q

Normovolemic Hemodolution crystalloid and colloid to blood ratios

A

3ml:1ml and 1ml:1ml

56
Q

Cell saver vaccum setting

A

150 Torr

57
Q

B3 phenotype

A

Taiwan (mf on forward typing)

58
Q

Acquired B phenotype associated with these bacteria (2)

A
  1. Proteus vulgaris

2. E. coli

59
Q

TH-1

A

binds only A1 cells (like Dilochos Biflorus)

60
Q

of D antigens in weak D

A

200-5,000

61
Q

1st CXM

A

1907 Ottenberg

62
Q

1st animal to human transfusion

A

1667 Jean Baptiste Denis

63
Q

1st human to human transfusion

A

1818 James Blundell

64
Q

% of directed donor units used

A

70%

65
Q

Donor testing for therapeutic T cells

A

7 days before or after collection

66
Q

Donor testing for HPC and oocytes

A

30 days before up to 7 days after collection

67
Q

HLA platelet matching: A

A

4 antigens matched

68
Q

HLA platelet matching: BIU

A

3 antigens matched, one untested (homozygous)

69
Q

HLA platelet matching: BIX

A

3 antigens matched, one in same crossreactive group

70
Q

HLA platelet matching: B2U

A

2 antigens matched, 2 unknown (homozygous)

71
Q

HLA platelet matching: B2UX

A

2 antigens matched, 1 unknown (homozygous), one in same crossreactive group

72
Q

HLA platelet matching: B2X

A

2 antigens matched, 2 in same crossreactive groups

73
Q

C

A

1 antigens mismatched

74
Q

D

A

2 antigens mismatched

75
Q

Granulocyte lifespan - native and with G-CSF stimulation

A

8 hrs and 24 hrs

76
Q

Contraindication to 8-MOP

A

Aphakia (absense of lenses)

77
Q

Freq of DHTR

A

1/2000-5000

78
Q

Freq of DSTR

A

1/3000

79
Q

Culex pipens vector

A

WNV

80
Q

Normal D antigen

A

10,000 - 3,000

81
Q

Lu null

A

poikilocytosis

82
Q

Duffy

A

chemokine receptor

83
Q

Knoops

A

C3b/C4b, CR1 receptor

84
Q

Indian

A

lymphocyte homing receptor/hyaloronan receptor/CD44 adhesion, polio

85
Q

Kidd

A

urea transporter

86
Q

Colton

A

water transporter (auaporin)

87
Q

Diego/Wright

A

Band 3 ion exchange

88
Q

Chido/Rodger

A

(C4B/C4A)

89
Q

Cromer

A

Decay acceleratin factor (DAF) CD55/E. coli, coxsackie, echovirus

90
Q

Gerbich

A

Glycophorin C&D

91
Q

Duffy, Cad, MNS

A

Plasmodium

92
Q

Anton (AnWj)

A

H. influenzae

93
Q

In-vitro hemolysis in antibody testing

A

I, A, B, Le(a/b), JK(a/b), p, pPk, Vel

94
Q

Brendemon phenomen

A

transient Le(a-b-) phenotype during pregnancy and development of anti-Le antibody

95
Q

Color of AHG reagent

A

Green

96
Q

Color of Anti-d reagent

A

Gray

97
Q

P1 phenotype antigens

A

P1,P,Pk

98
Q

P1 phenotype antibodies

A

None

99
Q

P2 phenotype antigens

A

P,Pk

100
Q

P2 phenotype antibodies

A

anti-P1

101
Q

Pk phenotype antigens

A

Pk

102
Q

Pk phenotype antibodies

A

anti-P

103
Q

p phenotype antigens

A

None

104
Q

p phenotype antibodies

A

anti-P1,P,Pk

105
Q

Allowable plasma loss in one year for 100-175 lb donor

A

12 L

106
Q

Allowable plasma loss in one year for >175 lb donor

A

14.4 L

107
Q

Frequent plasmapheresis donor - required serum protein

A

> 6.0 g/dL

108
Q

Post-deglyceralization RBC recovery

A

> 80%

109
Q

Post-leukoreduction RBC recovery

A

> 85%

110
Q

Expiration of deglyceralized cells in closed system

A

14 days at 1-6C

111
Q

Leukoreduction of 5 x 10^6 helps to prevent

A

CMV transmission and HLA immunization

112
Q

Leukoreduction of 5 x 10^8 helps to prevent

A

FNHTR

113
Q

Percentage of wasted autologous units

A

30%

114
Q

CPDA rejuvenation

A

w/in 6 days of expiration or 3 days after expiration in a closed system