Histo-/Cytologiske metoder (farvning, LM, EM) Flashcards

1
Q

Øjets opløsningsevne

A

0,2 mm

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2
Q

Lysmikroskops opløsningsevne

A

0,2 µm

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3
Q

Electronmicrosope opløsningsevne

A

0,2 nm

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4
Q

Pæparation af væv til LM (trin-for-trin)

A
Udtagning af væv (biopsi)
Fiksering (fx formalin)
Paraffinstøbning
Skæring
Farvning
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5
Q

Acidofili

A

Sure farvestoffer - Binder til positivt ladede strukturer (fx cytoplasmiske proteiner)

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6
Q

Basofili

A

“basiske” farvestoffer - Binder til negativt ladede strukturer (fx RNA DNA)

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7
Q

PAS farvning binder til?

A

Glykogen og glykoproteiner fx i muccin (Goblet cells)

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8
Q

VGH farvning binder til (Gul og rød farve)

A

Van Gieson farver cytoplasma gult og kollagen rødt.

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