Lecture 2: Antibody Function Flashcards
Which of these use Fc receptors?
What Igs are involved?
- Ag-specific receptor (BCR)
- Agglutination
- Neutralization
- Complement activation
- Opsonization
- ADCC
- Mast cell activation
Opsonization - IgG
ADCC - IgG
Mast cell activation - IgE
Which antibody isotype(s) are primary responsible for the following function?
Explain what this term means.
Ag-specific receptor (BCR)
Ag-specific receptor (BCR) - all isotypes
Which antibody isotype(s) are primary responsible for the following function? Explain what this term means.
Agglutination
Agglutination - IgM
Which antibody isotype(s) are primary responsible for the following function?
Neutralization
Neutralization - IgG and IgA
Which antibody isotype(s) are primary responsible for the following function? Explain what this term means.
Complement activation
Complement activation - IgM and IgG
Which antibody isotype(s) are primary responsible for the following function? Explain what this term means.
Opsonization
Opsonization - IgG
Which antibody isotype(s) are primary responsible for the following function? Explain what this term means.
ADCC
ADCC - IgG
Which antibody isotype(s) are primary responsible for the following function? Explain what this term means.
Mast cell activation
Mast cell activation - IgE
In multiple myeloma, what cells are malignant? What results from this?
Multiple Myeloma - malignant plasma cells
- excessive production of mAb
- normal hematopoiesis is disrupted
- anemia, low WBC counts, infection
What is the major serum protein?
albumin
g-globulin is found in the __.
It is mostly composed of __.
It appears on a gel as a __.
g-globulin: in the serum
mostly composed of antibodies (immunoglobulins)
It appears on a gel as a smear. Lots of Ig types.
What is a CDR? Where is it?
What does it do?
How many does a single Ab have?
CDR = Complementarity Determining Region (hypervariable region)
Where: at the end of the variable regions
Purpose: For Ag specificity
How many: 3 per H, 3 per L: 6 total
Where are there disulfide bonds in Ab?
Disulfide bonds
- between heavy chains
- between heavy and light chains
What does the Fab region do? What is it made of?
Fab: Ag binding region
1 L, 1 part of H
What is the Fc region? What does it do?
The Fc region is the constant region
Activate complement, opsonization, etc
