ECM as a Scaffold Material Flashcards
1
Q
ECM scaffold current applications
A
Repair of musclotendinous structures
Lower urinary tract reconstruction
Esophageal replacement
2
Q
ECM molecules?
A
Large mulitmodal regulate cell functions change conformation under mechanical load mimicked by small peptides remain attached to cells after isolation
3
Q
ECM composition
A
Collagen (>90%) Fibronectin (cell adhesion ligands) Laminin GAGs GFs
4
Q
Why is ECM a good scaffold?
A
Inherent ability to respond to environmental cues
5
Q
Decellularisation process?
A
Delamination
Physical, chemical & enzymatic methods to remove cellular material
6
Q
ECM common sources?
A
Small intestine, urinary bladder, dermis
xenogenic (conserved well over species)
7
Q
ECM sterilisation
A
gamma, electron, EO
shrink temperature of collagen, changes in ultrastructure and surface
8
Q
ECM degradation
A
degradation products can be biologically active (antimicrobial, GFs)
can promote constructive tissue remodelling
