Lab Practical: Oocyte retrieval

Question 1

What size of follicle is generally considered to be mature

Answer 1

between 15mm-20mm

Question 2

What are the main risks involve in an oocyte recovery

Answer 2

pelvic abscess- more likely in women with endometrioma of if bowel is damaged
damage surrounding structures e.g. bowel
bleeding- internal iliac vessels
risk of no eggs collected
problems with sedation e.g. maintaining airway, hypotension
pain
OHSS

Question 3

What factors may affect the oocyte quality and therefore must be controlled in the lab

Answer 3

pH- done by maintaining C02 levels
osmolarity
temperature

Question 4

how many mature follicles need to be seen before egg retrieval is scheduled?

Answer 4

three at 17mm
or two at 18mm
variations around this though

Question 5

where the needle inserted to to get to the ovary

Answer 5

lateral fornix of vagina

Question 6

what are the layers of the ovary

Answer 6

peritoneum
germinal layer eg. tunica albigunea
ovarian cortex
medulla

Question 7

how long is luteal support given for

Answer 7

until 8 weeks of pregnancy