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Endemic Oral Health > Caries, Micro Dev & Biofilms > Flashcards

Caries, Micro Dev & Biofilms Flashcards

1
Q

what kind of growth does planktonic growth undergo

A

vegetative

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2
Q

what is planktonic growth

A

single bac suspended in media
- rich or nutrient limiting
ideal conditions

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3
Q

what is th density growth of planktonic growth

A

low to high usually exponential

classic growth phase, lag, exponential, stationary and decline

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4
Q

what is the limited contact/ co aggregation impact on planktonic growth

A

cells should sep individually

starvation can sec material or alter growth to form raft and develop biofilm

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5
Q

what si the series of events in planktonic growth

A 
planktonic bac approach surface 
contact & attach
adherence and changes cell surface 
metab activity changes enviro 
quorum seeing and dev 
mature biofilm = new planktonic cells
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6
Q

what is F nucelatum involved in

A

plaque
perio disease
still birth
cancer

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7
Q

what is one of key issues in plaque

A

adhesive surface able to adhere

auto aggregation

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8
Q

what is the sensory input fro prokaryotic dev

A

starvation

high pop density

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9
Q

what is the response/enters dev pathway

A

biofilm form
competence
motility and chemotaxis
endospore form

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10
Q

give an example of developmental pathway

A

transition of planktonic growth to biofilm

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11
Q

what is quorum sensing

A

population density

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12
Q

what does b subtilise do

A

create spores

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13
Q

what gene influences biofilm formation

A

EzrA

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14
Q

what is the b subtilise cycle growth

A 
dormant spore
germination 
vegetative stare 
sporulation 
lyse 
spore again
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15
Q

what is the control of gene expression dept on

A

dna binding protein
sigma factor
RNA polymerase

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16
Q

what s the phosphorelay of B subtilis

A

a system for integrating environmental signals into entering developmental pathway

17
Q

what does networks of coordinated sigma factors result in

A

spore formation

18
Q

what is a biofilm

A

name given to microbial communities attached to a surface

complex ago of bac

19
Q

wha are the properties of a typical biofilm

A

spatially organised in a 3D Structure
bac cells enclosed in ECM
inc habitat range of indiv bac

20
Q

what are he purpose of a biofilm

A

cooperation behaviour
protection
microenvironment
structure

21
Q

what does a biofilm do in terms of habitat

A

extends habitat range

22
Q

what are the developmental stages of oral biofilm

A 
pellicle form
attachment
growth of individual bac
microbial succession and diversification 
climax community
23
Q

what re the key features assc with biofilm

A 
cells switched away from planktonic growth 
matrix ECM subs
nutrient gradients 
void spaces 
diff and dev
inter sp assc
24
Q

what is quorum sensing

A

process in which single celled organisms monitor their pop density by detecting the conc of small and diffusible signal mols produced by cells themselves

25
Q

what are some gm -ve and gm +ve

A

gm -ve - N acetyl homoserine lactones

gm +ve - oligopeptides

26
Q

what re some plaque formations

A

corn cob complex
cauliflower complex
hedgehog complex

27
Q

what is the sp plaque hypothesis

A

caries down to a limited NO of bac

28
Q

what si the non sp plaque hypothesis

A

caries outcome of overall activity of microbes

29
Q

what its the ecological plaque hypothesis

A

no sp mix of microorgs at a lesion

enviro change shifts proportion of subset of bac

30
Q

what is a causative situ fro caries

A

high?frequent sugar intake favours acidgmenic?acidoduric bacteria

31
Q

do all surfaces covered by biofilm = caries

A

no

32
Q

what are some prevention strategies

A 
oral hygiene 
diet 
fissure sealing 
fluoride 
nutrient control 
control of biofilm pH
control redox potential 
novel strategies

Endemic Oral Health (7 decks)

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