Factors Affecting Enzyme Action

Question 1

What is used to measure the progress of an enzyme-catalysed reaction?

Answer 1

Time-course

Question 2

What shows that all the substrate has been used in a graph showing enzyme reaction?

Answer 2

Graph line flattens out

Question 3

What does an increase in temperature do to enzyme reaction rate?

Answer 3

Increases collisions

Question 4

Does the rate of reaction increase or decrease as the temperature increases?

Answer 4

Increase

Question 5

At what temperature do enzymes begin denaturing in humans?

Answer 5

45 degrees Celsius

Question 6

What happens when an enzyme starts denaturing?

Answer 6

Bonds break and active site may change

Question 7

At what temperature do enzymes denature?

Answer 7

60 degrees Celsius

Question 8

Is denaturing permanent or reversible?

Answer 8

Permanent

Question 9

Name one use of enzymes in the home in cleaning

Answer 9

Biological washing powder

Question 10

What can cause the body temperature to change and denature enzymes?

Answer 10

Illness

Question 11

What does a change in pH do to enzymes?

Answer 11

Changes the amino acids that make up the active site

Question 12

Is the change in pH is significant enough what happens?

Answer 12

Bonds break and tertiary structure changes. Active site changes shape

Question 13

What does a low enzyme concentration do to the reaction?

Answer 13

Too few enzymes to substrate molecules so rate of reaction is low

Question 14

What happens in an intermediate enzyme concentration?

Answer 14

The substrate molecules can all occupy an active site and the ears of reaction is increased

Question 15

What happens when there is a high enzyme concentration?

Answer 15

More enzyme that substrate so rate of reaction doesn’t increase past a certain point

Question 16

What happens if there’s a low substrate concentration?

Answer 16

Too few substrate molecules for all available active sites