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Bacteriology Exam 2 > Escherichia coli > Flashcards

Escherichia coli Flashcards

1
Q

Specifics

A

Lactose positive
oxidase negative
motile rods
O antigen 26, K capsule 60 (B6), F fimbrae (41) and H flagella (11)

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2
Q

E. coli in pigs

A 
ETEC
VTEC
EPEC
UTI
MMA
Septicemia
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3
Q

ETEC Virulence factors neonatal

A

F5, F6, F41
non hemolytic
*everything passed neonatal is hemolytic

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4
Q

ETEC Virulence <4 weeks

A

F4, LT, EAST1

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5
Q

ETEC Virulence factors Weaning diarrhea

A

F4, F18, LT, EAST1

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6
Q

F4 Adhesion factor

A

pigs only, on plasmids,

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7
Q

F5 Adhesion factor

A

pigs and bovine, only receptor for very young animals (neonatal), T dependent expression

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8
Q

F6 Adhesion factor

A

extra chromosonal, neonatal

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9
Q

F41 Adhesion factor

A

pigs and bovine, chromosomal

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10
Q

F18 Adhesion factor

A

Edema disease

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11
Q

ETEC Heat labile toxin

A

high molecular weight, strong Ag and used to make vaccines

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12
Q

ETEC Heat stable toxin

A

low molecular weight, little antigenic

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13
Q

ETEC Clinical signs

A

watery diarrhea, very smell feces, dehydration

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14
Q

ETEC pathogenesis

A

ascend from lower bowel or oral uptake and then colonizes small intestine

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15
Q

ETEC epidemiology

A

infection get from intestine of healthy sows
equilibrium between maternal immunity and infection pressure
low hygiene

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16
Q

ETEC Tx and Prev

A

hydration
antimicrobials
vaccinate sows

17
Q

VTEC in pigs age affect

A

<3 weeks after weaning

18
Q

VTEC Clinical signs pig

A

Edema disease
swollen eye lids
hoarse voice
diarrhea with mixed and ETEC and VTEC

19
Q

VTEC virulence factor in pig

A

F18

Exotoxin- VT2e and STX2e media necrosis

20
Q

VTEC Prev and treatment in pigs

A

Affected pigs: fast to eliminate and then antimicrobial
Non affected pigs: antimicrobials and increase in 2-3 days
Prevent add zinc oxide

21
Q

EPEC (enteropathogenic) in pigs

A

rare
weaning pigs - diarrhea
virulence factor T3SS (needle and injection)
Diagnostics- Isolation and PCR (Eae- intimin gene)

22
Q

UTI in pigs

A

CS: anorexia, vaginal exudate

Tx/Prev: culling due to fertility problems, antibitotics for 3 weeks

23
Q

MMA in pigs

A

48 hours after farrowing
post partum dysgalactiae syndrome- no milk production, colostrum and septicemia
Edx: hereditary, hormonal, feed, infectious agents

24
Q

Septicemia in pigs

A

secondary to enteric infection

rarely primary

25
Q

ETEC in cows

A

age affected less than 3 days
Dx: ELISA, culture and virulence factors (agglutination/PCR)
Virulence factors: F5, F41, CS31A, STa, Stb
Therapy: separate sick animals, hydration, antibiotics extremely high resistance, vaccinate dams, colostrum

26
Q

EPEC (enteropathogenic)
EHEC (enterohemorrhagic)- Zoonotic
in cow

A 
Age 1-8 weeks 
CSS: mucoid diarrhea with/without blood 
mainly large intestine 
Therapy: antibiotics 
Virulence factors: 
EPEC- initimin eae, EHEC intimin eae + VT1 and VT2
27
Q

Mastitis in cow

A

CS: udder swelling, redness, lesions
prev/tx: hygiene and systemic Antibiotics
no known virulence factors

28
Q

Septicemia in cow

A

CS: hyperacute- acute mortality and shock,
acute- general symptoms, fast mortality,
slower- organ localization: polyarthiritis, pneumonia, pleuritis, meningo- encephalitis
Pathogenesis:
PO–> lymph tissue–> septicemia / umbilical cord–>septicemia

29
Q

E. coli in dogs and cats (ETEC, EPEC, VTEC, EIEC)

A

Frequent with other pathogens

Virulence factor CNF1- Ecoli in puppies

30
Q

Cysitis/ pyometra

A

Cystitis inflammation in bladder, pyometra uterine infection
cats seldom get cystitis, high osmolarity of urine
Virulence factors: F12, F13 (associated with human UTI)
Specific (o2,o4,o6,o83)

31
Q

E. coli in birds

Clinical signs and Pathogensis

A

most important bacterial disease in broilers and layers
Neonatal colibacillosus –> contamination of egg, neonatal or manipulation of chick that leads to yolk rest infection called omphalitis–> sepsis/death/retardation
Respiratory colibacillosus–> excretion leads to increase of infection pressure–> inhaled–> cause infection or can damage resp epithelium
Peritonitis–> decrease egg production

32
Q

Ecoli in birds- prevention

A

hygiene, decrease stress, control temp, vaccines, difficult to treat

33
Q

Ecoli is broilers

A

Serotype O78, O2
CS: scabby hips/necrotic dermatitis in broilers
Use feathers for diagnosis, usually at slaughter
Prev: low density of animals

34
Q

E coli in rabbits

RPEC/EPEC

A

virulence factor intimin eae
Dx- isolate and identify serotype
Tx- antibiotics

Bacteriology Exam 2 (15 decks)

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