Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology Path > Media > Flashcards

Media Flashcards

1
Q

Blood agar plate (BAP)

A

Cultivation of most organisms, determination of hemolytic reactions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Chocolate agar

A
  • Blood is heated until lysed

- cultivation of Haemophilus, pathogenic Neisseria, and nutritionally-deficient Strep

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Columbia-nalidixic acid agar (CNA)

A

selective isolation of Gram+ cocci; inhibits most Gram- organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Mannitol salt agar

A

isolate S. aureus from mixed culture; inhibits most Gram- organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

MacConkey agar

A
  • isolation and differentiation of lactose fermenting and non-lactose fermenting enteric bacilli
  • lactose fermenters are red/pink, while non-lactose fermenters are clear
  • Gram+ and fastidious organisms inhibited by bile salts and crystal violet
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Lactose fermenters (CEEK)

A

Citrobacter, E. coli, Enterobacter, Klebsiella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Eosin methylene blue agar (EMB)

A 
isolation and differentiation of Enterobacteriaceae and Gram- rods
o	Green sheen: characteristic of E. coli
o	Pigmented (black/purple): lactose fermenters
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Xylose lysine deoxychocolate agar (XLD)

A

isolation and differentiation of salmonella and shigella from other normal stool flora

  • Red with black center: salmonella and H2S producers
  • Red: shigella (cannot ferment xylose)
  • Yellow/orange: normal colonic flora
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Hektoen-enteric agar (HE)

A

isolation and differentiation of salmonella and shigella from other normal stool flora

  • Green w/ black center: Salmonella
  • Green: shigella
  • Salmon pink/orange: lactose fermenters
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Salmonella-shigella agar (SS)

A

isolation and differentiation of salmonella and shigella from other normal stool flora

  • Colorless with black center: salmonella
  • colorless: shigella
  • red: lactose fermenters
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Thayer-Martin agar (MTM)

A

chocolate agar with vanc, colistin and nystatin

selective for N/ gonorrhea and meningitidis; suppresses growth of saphrophytic Neisseria species

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

GC-Lect agar

A

used for pathogenic Neisseria; permits growth of some vanc-sensitive Neisseria that are inhibited by MTM agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Buffered charcoal yest extract agar (BCYE)

A

growth of nocardia and legionella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Regan Lowe

A

isolation of Bordetella pertussis and parapertussis; inhibits normal flora of nasopharynx and yeast/fungi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Sabouraud dextrose agar (SDA)

A

General fungal media. Inhibits growt of some bacteria due to high glucose content and low pH.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Potato Flake Agar (PFA)

A

Primary recovery media for saprophytic (living on dead organic matter) and pathogenic fungi

17
Q

Yeast ChromAgar

A

Selective medium for isolation and presumptive identification of yeast and filamentous fungi. Candida species different colors.

18
Q

Potato Dextrose Agar (PDA)

A

Used for prep of microslide culture and sporulation of dermatophytes. Demontrates pigment by T. rubrum

19
Q

Dermatophyte Test medium (DTM)

A

SDA with added cyclohexamide to inhibit saprotrophic growth. Antibiotics to inhibit bacterial growth and phenol red as pH indicator (agar turns red in presence of dermatophytes)

20
Q

Cycloheximide agar

A

Primary recovery media for dermatophytes and dimorphic fungi. Contains chloramphenicol and cyclohexamide to inhibit bacteria and saprophytic fungi respectrively.

21
Q

Brain-Heart-Infusion agar

A

enriched media for fastidious fungi.

22
Q

Lowenstein-Jensen Media

A

semisolid egg-based media
contains malachite green
used for MTB and NTMs

23
Q

MGIT Broth media

A

modified middlebrook broth medai

antiobiotic cocktail added to suppress growth of other bacteria

24
Q

C. diptheria media

A

Modified Tinsdale media

Cystine tellurite blood agar

25
Q

quad plate

A

Used to grow H. influenza. Has factors X (hemin) and V (NAD) required for H. flu growth.

26
Q

CCFA media (cycloserine cefoxitin fructose)

A

selective for C. difficile, yellow ground-glass colonies

Microbiology Path (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions