DNA replication

Question 1

What is responsible for removing erroneously placed nucleotides?

Answer 1

3’-5’ exonuclease

Question 2

Polymerases have a P and E site. What is their respective roles?

Answer 2

P site: polymerization

E site: editing

Question 3

What is the role of MutS and MutL?

Answer 3

Detects mismatched base, cuts at it and at the next nick of the sequence

Question 4

Define DNA polymerase I

Answer 4

fills the gaps during DNA repair

Question 5

DNA polymerase II role

Answer 5

Repairs fork stalled by DNA damage

Question 6

DNA polymerase III role

Answer 6

DNA replication

Question 7

How long are RNA primers

Answer 7

10 nucleotides

Question 8

DNA helicase role

Answer 8

Unwinds DNA

Question 9

Clamp role

Answer 9

Keeps DNA polymerase on the strand synthesizing

Question 10

Association between DNA helicase and DNA primase is called…

Answer 10

A primosome

Question 11

Role of DNA topoisomerase

Answer 11

Makes temporary breaks in DNA backbone to relieve stress of unwinding

Question 12

DNA primase in eukaryotes is made of a complex of subunits named….

Answer 12

DNA polymerase alpha/primase

Question 13

What DNA polymerase is used on the leading strand?

Answer 13

epselon

Question 14

What DNA polymerase is used on the lagging strand?

Answer 14

delta

Question 15

What is the last two dimers to be added to the histone, therefore the first to come off in destabilization?

Answer 15

H2A-H2B dimer

Question 16

What enzyme loads H2A-H2B dimer back into the nucleosomes?

Answer 16

NAP-1

Question 17

What enzyme loads H3-H4 dimer back into the nucleosomes?

Answer 17

CAF-1