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Bio 196 L > Enzyme Activity > Flashcards

Enzyme Activity Flashcards

1
Q

cellobiase

A
  • Enzyme that catalyzes the hydrolysis of glucose B 1-4 glycosidic bonds
  • Crucial to life cycles of organisms that obtain energy by hydrolyzing cellulose
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2
Q

cellulose/cellobiose

A

Most prominent structural carbohydrate of plants

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3
Q

ethanol

A

fuel made by breaking down cellulose into glucose

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4
Q

p-Nitrophenyl glucopryanoside (pNG)

A
  • Artificial substrate
  • Produces a colored product when exposed to a solution that is basic in pH.
  • Absorbs light at the wavelength of 410 nm
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5
Q

spectrophotometer

A
  • Transmits beams of light through a substance to measure how much to the light is absorbed.
  • The darker the color the more product is formed
  • Rate of reaction can be measured
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6
Q

Brownian motion

A

Randomly moving molecules

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7
Q

Vmax

A
  • All enzymes in solution are actively catalyzing the formation of product
  • Add more enzymes to increase
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8
Q

Environmental factors that effect enzymes

A
  • Temperature
  • pH
  • Enzyme concentration
  • Substrate concentration
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Bio 196 L (9 decks)

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