Microbiological methods

Question 1

why are microbiological criteria needed?

Answer 1

determine which organisms are considered hazards

Question 2

What are standards

Answer 2

• Document “that provides, specifications, guidelines or charcterstics that can be used consistently.
• ISO standards – 3 years to develop
• Inside a standard – detailed description, media composition and result expression guide

Question 3

Detection method

Answer 3

enrichment  selective enrichment  isolation  purification  serological confirmation  biochemical confirmation

Question 4

Advances in traditional microbiology

Answer 4

• Chromogenic media – aids isolation of target, microorganisms and shorten analysis time
• Petrifilms – no prep required, no use of spreaders.
• Automation in microbiology – increase precision and accuracy

Question 5

Rapid methods

Answer 5

• Bioluminescence – ATP
• Flow cytometry – characterises cells suspended in liquid based upon light absorption and scattering
• Impedimetry – measures changes in electric properties conductivity, impedence or capacitance
• Direct epiflurscent filter technique
• Immunological methods – antibodies binds to a specific pathogen to enable detection in products – method ELISA