6A

Question 1

Cultivation indépendant methods

Answer 1

DNA can be amplified and sequenced by PCR

Question 2

Special slide with etched grid

Answer 2

Direct count

Question 3

Microbial constorita

Answer 3

some microbes are too accostumed to living with neihbors

Question 4

FISH

Answer 4

Flourescent In Stitu Hybridization

Question 5

Known volume is added onto the grid and counted with a light microscope

Answer 5

direct count

Question 6

problem if microbe cannot be cultured

Answer 6

Viable count

Question 7

Dilutions are made and added to a suitable media and turbidity yeilds MPN

Answer 7

viable count

Question 8

lag phase

Answer 8

cells synthesizing new components

Question 9

replenish, adaption, various length

Answer 9

lag phase

Question 10

cheap/fast/easy

cannot differentiate between living and non living cells

Answer 10

direct count

Question 11

log/exponential phase

Answer 11

rate of growth and cell division is constant and maximal

Question 12

Stationary phase

Answer 12

active cells stop producing, or the rate of cells being made and cells dying is equal.

Question 13

stationary phase is due to:

Answer 13

Nutrient limitation, limited oxygen, waste, and a max population reached

Question 14

generation time

Answer 14

time to double population

Question 15

population is most uniform

Answer 15

log/exponential phase

Question 16

growth rate

Answer 16

number of generations/ time

Question 17

growth yeild

Answer 17

maximum population density and/or amount of cellular material produced by culture

Question 18

continuous culture

Answer 18

continual provision of nutrients, continual removal of waste.